Labyrinthopeptins as virolytic inhibitors of respiratory syncytial virus cell entry.


Journal

Antiviral research
ISSN: 1872-9096
Titre abrégé: Antiviral Res
Pays: Netherlands
ID NLM: 8109699

Informations de publication

Date de publication:
05 2020
Historique:
received: 25 10 2019
revised: 03 02 2020
accepted: 12 03 2020
pubmed: 22 3 2020
medline: 2 4 2021
entrez: 22 3 2020
Statut: ppublish

Résumé

Acute lower respiratory tract infections (ALRI) caused by respiratory syncytial virus (RSV) are associated with a severe disease burden among infants and elderly patients. Treatment options are limited. While numerous drug candidates with different viral targets are under development, the utility of RSV entry inhibitors is challenged by a low resistance barrier and by single mutations causing cross-resistance against a wide spectrum of fusion inhibitor chemotypes. We developed a cell-based screening assay for discovery of compounds inhibiting infection with primary RSV isolates. Using this system, we identified labyrinthopeptin A1 and A2 (Laby A1/A2), lantibiotics isolated from Actinomadura namibiensis, as effective RSV cell entry inhibitors with IC

Identifiants

pubmed: 32197980
pii: S0166-3542(19)30614-X
doi: 10.1016/j.antiviral.2020.104774
pii:
doi:

Substances chimiques

Antiviral Agents 0
Bacteriocins 0
labyrinthopeptin A1, Actinomadura namibiensis 0
labyrinthopeptin A2 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104774

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The antiviral activity of Laby A1/A2 against a broad spectrum of enveloped viruses has been patented (PCT/EP2016/078143). Otherwise, no competing financial interests exist.

Auteurs

Sebastian Blockus (S)

Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany.

Svenja M Sake (SM)

Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany.

Martin Wetzke (M)

Department for Pediatric Pneumology, Allergy and Neonatology, Hannover Medical School, Hannover, Germany; German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany.

Christina Grethe (C)

Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany.

Theresa Graalmann (T)

Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany; Clinic for Immunology and Rheumatology, Hannover Medical School, Hannover, Germany.

Marina Pils (M)

Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.

Ronan Le Goffic (R)

Unité de Virologie et Immunologie Moléculaires, INRA, Université Paris Saclay, Jouy-en-Josas, France.

Marie Galloux (M)

Unité de Virologie et Immunologie Moléculaires, INRA, Université Paris Saclay, Jouy-en-Josas, France.

Hans Prochnow (H)

Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany; German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany.

Katharina Rox (K)

Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany; German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany.

Stephan Hüttel (S)

Department Microbial Drugs, Helmholtz Centre for Infection Research, Braunschweig, Germany.

Zeljka Rupcic (Z)

Department Microbial Drugs, Helmholtz Centre for Infection Research, Braunschweig, Germany.

Bettina Wiegmann (B)

Lower Saxony Center for Biomedical Engineering, Implant Research and Development, Hannover Medical School, Hannover, Germany; Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany; Member of the German Centre for Lung Research (DZL), Hannover, Germany.

Ronald Dijkman (R)

Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Switzerland; Institute for Infectious Diseases, University of Bern, Switzerland.

Marie-Anne Rameix-Welti (MA)

UMR1173, Institute National de la Santé et de la Recherche Médicale (INSERM), Université de Versailles St. Quentin, Montigny-le-Bretonneux, France.

Jean-François Eléouët (JF)

Unité de Virologie et Immunologie Moléculaires, INRA, Université Paris Saclay, Jouy-en-Josas, France.

W Paul Duprex (WP)

University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

Volker Thiel (V)

Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Switzerland.

Gesine Hansen (G)

Department for Pediatric Pneumology, Allergy and Neonatology, Hannover Medical School, Hannover, Germany; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.

Mark Brönstrup (M)

Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany; German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany.

Sibylle Haid (S)

Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany. Electronic address: sibylle.haid@twincore.de.

Thomas Pietschmann (T)

Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, Hannover, Germany; German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Germany; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany. Electronic address: thomas.pietschmann@twincore.de.

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Classifications MeSH