The neuroprotective effect of NeuroAid on morphine-induced amnesia with respect to the expression of TFAM, PGC-1α, ΔfosB and CART genes in the hippocampus of male Wistar rats.
Amnesia
/ chemically induced
Animals
Apoptosis
/ drug effects
Behavior Observation Techniques
Behavior, Animal
/ drug effects
Disease Models, Animal
Drugs, Chinese Herbal
/ administration & dosage
Hippocampus
/ drug effects
Humans
Injections, Intraperitoneal
Male
Morphine
/ administration & dosage
Nerve Tissue Proteins
/ metabolism
Neuroprotective Agents
/ administration & dosage
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
/ metabolism
Proto-Oncogene Proteins c-fos
/ metabolism
Rats
Rats, Wistar
Transcription Factors
/ metabolism
CART
Morphine
NeuroAid
PGC-1α
TFAM
ΔfosB
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
05 Jun 2020
05 Jun 2020
Historique:
received:
04
01
2020
revised:
16
02
2020
accepted:
16
03
2020
pubmed:
22
3
2020
medline:
2
6
2020
entrez:
22
3
2020
Statut:
ppublish
Résumé
Morphine is a natural alkaloid which derived from the opium poppy Papaver somniferum. Many studies have reported the effect of morphine on learning, memory and gene expression. CART (cocaine-amphetamine regulated transcript)is an important neuropeptide which has a critical role in physiological processes including drug dependence and antioxidant activity. ΔfosB is a transcription factor which modulates synaptic plasticity and affects learning and memory. TFAM (the mitochondrial transcription factor A) and PGC-1α (Peroxisome proliferator-activated receptor γ coactivator-1α) are critically involved in mitochondrial biogenesis and antioxidant pathways. NeuroAid is a Chinese medicine that induces neuroprotective and anti-apoptotic effects. In this research, we aimed to investigate the effect of NeuroAid on morphine-induced amnesia with respect to the expression of TFAM, PGC-1α, ΔfosB and CART in the rat's hippocampus. In this study, Morphine sulfate (at increasing doses), Naloxone hydrochloride (2.5 mg/kg) and NeuroAid (2.5 mg/kg) were administered intraperitoneal and real-time PCR reactions were done to assess gene expression. The results showed, morphine impaired memory of step-through passive avoidance, while NeuroAid had no effect. NeuroAid attenuated (but not reversed) morphine-induced memory impairment in morphine-addicted rats. Morphine increased the expression of PGC-1α and decreased the expression of CART. However, NeuroAid increased the expression of TFAM, PGC-1α, ΔfosB and CART. NeuroAid restored the effect of morphine on the expression of CART and PGC-1α. In conclusion, morphine impaired memory of step-through passive avoidance and NeuroAid attenuated this effect. The effect of NeuroAid on morphine-induced memory impairment/gene expression may be related to its anti-apoptotic and neuroprotective effects.
Identifiants
pubmed: 32198124
pii: S0378-1119(20)30270-5
doi: 10.1016/j.gene.2020.144601
pii:
doi:
Substances chimiques
Drugs, Chinese Herbal
0
Fosb protein, rat
0
Nerve Tissue Proteins
0
Neuroaid
0
Neuroprotective Agents
0
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
0
Ppargc1a protein, rat
0
Proto-Oncogene Proteins c-fos
0
Tfam protein, rat
0
Transcription Factors
0
cocaine- and amphetamine-regulated transcript protein
0
Morphine
76I7G6D29C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144601Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.