Reproductive tract extracellular vesicles are sufficient to transmit intergenerational stress and program neurodevelopment.
Adolescent
Animals
Cell Culture Techniques
Epididymis
/ metabolism
Epigenesis, Genetic
Epigenomics
Extracellular Vesicles
/ metabolism
Female
Germ Cells
Histones
Humans
Male
Mice
Mice, Inbred C57BL
MicroRNAs
/ metabolism
Nanoparticles
Nervous System
/ growth & development
Proteomics
Reproduction
/ physiology
Sperm Maturation
/ genetics
Spermatogenesis
/ genetics
Spermatozoa
/ metabolism
Stress, Physiological
Testis
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
20 03 2020
20 03 2020
Historique:
received:
02
04
2018
accepted:
27
02
2020
entrez:
22
3
2020
pubmed:
22
3
2020
medline:
18
7
2020
Statut:
epublish
Résumé
Extracellular vesicles (EVs) are a unique mode of intercellular communication capable of incredible specificity in transmitting signals involved in cellular function, including germ cell maturation. Spermatogenesis occurs in the testes, behind a protective barrier to ensure safeguarding of germline DNA from environmental insults. Following DNA compaction, further sperm maturation occurs in the epididymis. Here, we report reproductive tract EVs transmit information regarding stress in the paternal environment to sperm, potentially altering fetal development. Using intracytoplasmic sperm injection, we found that sperm incubated with EVs collected from stress-treated epididymal epithelial cells produced offspring with altered neurodevelopment and adult stress reactivity. Proteomic and transcriptomic assessment of these EVs showed dramatic changes in protein and miRNA content long after stress treatment had ended, supporting a lasting programmatic change in response to chronic stress. Thus, EVs as a normal process in sperm maturation, can also perform roles in intergenerational transmission of paternal environmental experience.
Identifiants
pubmed: 32198406
doi: 10.1038/s41467-020-15305-w
pii: 10.1038/s41467-020-15305-w
pmc: PMC7083921
doi:
Substances chimiques
Histones
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1499Subventions
Organisme : NCI NIH HHS
ID : P01 CA196539
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM110174
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM092237
Pays : United States
Commentaires et corrections
Type : CommentIn
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