First European Haplotype of Echinococcus multilocularis Identified in the United States: An Emerging Disease?
Echinococcus multilocularis
Echinococcus multilocularis COX gene
alveolar echinococcus spectrum of disease
liver masses
parasitic infection
Journal
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
ISSN: 1537-6591
Titre abrégé: Clin Infect Dis
Pays: United States
ID NLM: 9203213
Informations de publication
Date de publication:
08 04 2021
08 04 2021
Historique:
received:
22
10
2019
accepted:
16
03
2020
pubmed:
22
3
2020
medline:
29
4
2021
entrez:
22
3
2020
Statut:
ppublish
Résumé
Echinococcus multilocularis is one of the most severe and lethal parasitic diseases of humans, most often reported in Europe and Asia. Only 1 previous case has been documented in the contiguous United States from Minnesota in 1977. European haplotypes have been identified in carnivores and domestic dogs as well as recently in patients in western and central Canada. We used immunohistochemical testing with the monoclonal antibody Em2G11 and a species-specific enzyme-linked immunosorbent assay affinity-purified antigen Em2, as well as COX1 gene sequencing. Using pathology, immunohistochemical staining, specific immunodiagnostic testing, and COX1 gene sequencing, we were able to definitively identify E. multilocularis as the causative agent of our patient's liver and lung lesions, which clustered most closely with the European haplotype. We have identified the first case of a European haplotype E. multilocularis in the United States and the first case of this parasitic infection east of the Mississippi River. Given the identification of this haplotype in Canada, this appears to be an emerging infectious disease in North America.
Sections du résumé
BACKGROUND
Echinococcus multilocularis is one of the most severe and lethal parasitic diseases of humans, most often reported in Europe and Asia. Only 1 previous case has been documented in the contiguous United States from Minnesota in 1977. European haplotypes have been identified in carnivores and domestic dogs as well as recently in patients in western and central Canada.
METHODS
We used immunohistochemical testing with the monoclonal antibody Em2G11 and a species-specific enzyme-linked immunosorbent assay affinity-purified antigen Em2, as well as COX1 gene sequencing.
RESULTS
Using pathology, immunohistochemical staining, specific immunodiagnostic testing, and COX1 gene sequencing, we were able to definitively identify E. multilocularis as the causative agent of our patient's liver and lung lesions, which clustered most closely with the European haplotype.
CONCLUSIONS
We have identified the first case of a European haplotype E. multilocularis in the United States and the first case of this parasitic infection east of the Mississippi River. Given the identification of this haplotype in Canada, this appears to be an emerging infectious disease in North America.
Identifiants
pubmed: 32198510
pii: 5810739
doi: 10.1093/cid/ciaa245
pmc: PMC8028098
doi:
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1117-1123Commentaires et corrections
Type : CommentIn
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
Références
PLoS Negl Trop Dis. 2012;6(10):e1877
pubmed: 23145198
Adv Parasitol. 2017;95:315-493
pubmed: 28131365
Parasite. 2014;21:73
pubmed: 25531581
J Parasitol. 1984 Oct;70(5):844
pubmed: 6512652
Parasitology. 1991 Aug;103 Pt 1:41-9
pubmed: 1945524
Emerg Infect Dis. 2018 Feb;24(2):230-235
pubmed: 29350139
Transplantation. 2003 Mar 27;75(6):856-63
pubmed: 12660515
Acta Trop. 2010 Apr;114(1):1-16
pubmed: 19931502
Parasitol Int. 2009 Dec;58(4):384-9
pubmed: 19651237
Curr Opin Infect Dis. 2003 Oct;16(5):437-44
pubmed: 14501996
Food Waterborne Parasitol. 2019 May 08;16:e00055
pubmed: 32095626
Parasitology. 2007 Jun;134(Pt 6):879-88
pubmed: 17306060
Adv Parasitol. 2017;95:65-109
pubmed: 28131366
Am J Trop Med Hyg. 1980 Nov;29(6):1340-55
pubmed: 7446824
JAMA. 1979 Mar 2;241(9):904-7
pubmed: 762867
Clin Microbiol Rev. 2004 Jan;17(1):107-35
pubmed: 14726458
Adv Parasitol. 2017;96:259-369
pubmed: 28212790
Am J Trop Med Hyg. 2015 Mar;92(3):477-8
pubmed: 25404078
Am J Trop Med Hyg. 2008 Aug;79(2):245-7
pubmed: 18689631
J Parasitol. 2000 Feb;86(1):75-7
pubmed: 10701567
N Engl J Med. 2019 Jul 25;381(4):384-385
pubmed: 31340100
Diagn Microbiol Infect Dis. 2017 Nov;89(3):185-190
pubmed: 28865743
Zoonoses Public Health. 2017 Sep;64(6):460-467
pubmed: 28012251
J Parasitol. 1983 Aug;69(4):790-1
pubmed: 6631648