Clinico-pathological correlation of optical fluorescence imaging in oral mucosal lesions.
autofluorescence
histomorphometric measurements
histopathological correlations
optical fluorescence imaging
oral mucosal lesions
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
revised:
22
02
2020
received:
20
10
2019
accepted:
15
03
2020
medline:
22
3
2020
pubmed:
22
3
2020
entrez:
22
3
2020
Statut:
ppublish
Résumé
This study aimed to identify clinical and pathological characteristics of oral mucosal lesions that may be predictive of optical autofluorescence imaging patterns. Clinical data and archival histopathological material were collected from patients who presented with at least one oral mucosal lesion and underwent assessment via conventional oral examination, optical autofluorescence imaging and histopathological analysis. An open-source digital pathology image analysis software was used to perform histomorphometric measurements. Classification and regression trees were used to determine histopathological characteristics most predictive of a clinical autofluorescence outcome. Histomorphometric features associated with tissue architecture, epithelial changes, inflammation and vasculature were found to be significantly associated with autofluorescence patterns. Diascopic fluorescence was found to be significantly predicted by lichenoid inflammation and was significantly associated with a diagnosis of oral lichen planus. Loss of autofluorescence with partial blanching was significantly associated with histopathological features noted in dysplastic and malignant lesions. This study provides evidence for the use of diascopic fluorescence as a technique to aid in clinical differentiation of benign inflammatory lesions from potentially malignant pathology. Based on the findings of this study, optical fluorescence imaging is a technique of added value in discernment of oral mucosal lesions, and our results support its clinical use.
Types de publication
Journal Article
Langues
eng
Pagination
1230-1239Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.
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