The effect of polyclonal activators on rabbit B cell antibody production.
Journal
Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
21
08
2019
revised:
20
12
2019
accepted:
13
03
2020
pubmed:
22
3
2020
medline:
12
9
2020
entrez:
22
3
2020
Statut:
ppublish
Résumé
Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production.
Identifiants
pubmed: 32199178
pii: S0034-5288(19)30863-X
doi: 10.1016/j.rvsc.2020.03.022
pii:
doi:
Substances chimiques
Oligodeoxyribonucleotides
0
alpha-Macroglobulins
0
polyclonal B cell activator
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
193-196Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.