Use of the reticulocyte channel warmed to 41°C of the XN-9000 analyzer in samples with the presence of cold agglutinins.
Autoimmune hemolytic anemia
Cold agglutinins
RET channel
Sysmex
XN-9000
Journal
Hematology, transfusion and cell therapy
ISSN: 2531-1387
Titre abrégé: Hematol Transfus Cell Ther
Pays: Brazil
ID NLM: 101725732
Informations de publication
Date de publication:
Historique:
received:
09
09
2019
revised:
25
11
2019
accepted:
09
01
2020
pubmed:
23
3
2020
medline:
23
3
2020
entrez:
23
3
2020
Statut:
ppublish
Résumé
The purpose of this study was to compare data obtained from the reticulocyte channel (RET channel) heated to 41°C with those obtained from impedance channel (I-Channel) at room temperature in the samples with the mean corpuscular hemoglobin concentration (MCHC)<370g/L and in samples with the MCHC>370g/L, in the presence of cold agglutinins. In this study, 60 blood samples (group 1) with the MCHC<370g/L (without cold agglutinins) and 78 blood samples (group 2) with the MCHC>370g/L (with cold agglutinins) were used to compare the two analytical channels of the XN-9000 analyzer in different preanalytical conditions. The parameters evaluated in both groups were the following: red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean cell volume (MCV), RBC-most frequent volume (R-MFV), mean hemoglobin concentration (MCH) and mean cellular hemoglobin concentration (MCHC). The results of this study showed an excellent correlation with both channels of the XN-9000 analyzer in samples with and without cold agglutinins, except for the MCHC. The bias between the values obtained in the I-channel and those obtained in the RET channel of both groups was insignificant and remained within the limits of acceptability, as reported by Ricos et al. for all considered parameters, except for MCHC. The presence of cold agglutinins in blood samples can be detected by a spurious lowering of the RBC count and by a spurious increase in the MCHC. The RET channel represents a great opportunity to correct the RBC count in a rapid manner without preheating. However, neither methodology can completely solve the residual presence of cold agglutinins in all samples, despite the MCHC values being < 370g/L.
Identifiants
pubmed: 32199923
pii: S2531-1379(20)30024-9
doi: 10.1016/j.htct.2020.01.004
pmc: PMC8211624
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
147-155Informations de copyright
Copyright © 2020 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier España, S.L.U. All rights reserved.
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