Flow-cytometry detection of fluorescent magnetic nanoparticle clusters increases sensitivity of dengue immunoassay.
Dengue fever
Diagnostic
Flow cytometry
Immunoassay
Nanoparticle
Paramagnetic
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
22 Apr 2020
22 Apr 2020
Historique:
received:
04
10
2019
revised:
03
01
2020
accepted:
04
02
2020
entrez:
24
3
2020
pubmed:
24
3
2020
medline:
15
12
2020
Statut:
ppublish
Résumé
We report a flow-cytometry based method capable of detecting a range of analytes by monitoring the analyte-induced clustering of magnetic and fluorescent nanoparticles with flow cytometry. Using the dengue viral antigen (NS1) as an example, antibodies were conjugated to magnetic and fluorescent nanoparticles in a sandwich immunoassay format. These nanoparticles formed clusters when NS1 was present in a sample and the cluster formation was directly proportional to the concentration of antigen. Simultaneous flow cytometry measurement of cluster size, as detected by the forward scatter channel, combined with fluorescence intensity led to a reduction in the assay background signal, resulting in improved analytical sensitivity. We were able to detect 2.5 ng mL
Identifiants
pubmed: 32200905
pii: S0003-2670(20)30162-8
doi: 10.1016/j.aca.2020.02.007
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Biomarkers
0
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85-91Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.