Interaction of an IκBα Peptide with 14-3-3.
Journal
ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658
Informations de publication
Date de publication:
17 Mar 2020
17 Mar 2020
Historique:
received:
22
12
2019
accepted:
19
02
2020
entrez:
24
3
2020
pubmed:
24
3
2020
medline:
24
3
2020
Statut:
epublish
Résumé
Inflammatory responses mediated by the transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB) play key roles in immunity, autoimmune diseases, and cancer. NF-κB is directly regulated through protein-protein interactions, including those with IκB and 14-3-3 proteins. These two important regulatory proteins have been reported to interact with each other, although little is known about this interaction. We analyzed the inhibitor of nuclear factor kappa B α (IκBα)/14-3-3σ interaction via a peptide/protein-based approach. Structural data were acquired via X-ray crystallography, while binding affinities were measured with fluorescence polarization assays and time-resolved tryptophan fluorescence. A high-resolution crystal structure (1.13 Å) of the uncommon 14-3-3 interaction motif of IκBα (IκBαpS63) in a complex with 14-3-3σ was evaluated. This motif harbors a tryptophan that makes this crystal structure the first one with such a residue visible in the electron density at that position. We used this tryptophan to determine the binding affinity of the unlabeled IκBα peptide to 14-3-3 via tryptophan fluorescence decay measurements.
Identifiants
pubmed: 32201828
doi: 10.1021/acsomega.9b04413
pmc: PMC7081424
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5380-5388Informations de copyright
Copyright © 2020 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
J Biol Chem. 2007 Feb 9;282(6):3766-77
pubmed: 17170118
Mol Cell Biol. 1996 Apr;16(4):1295-304
pubmed: 8657102
FEBS J. 2018 Nov;285(22):4196-4213
pubmed: 30281929
Biochemistry. 2008 Feb 12;47(6):1768-77
pubmed: 18181650
EMBO J. 2003 Mar 3;22(5):987-94
pubmed: 12606564
Biophys J. 2017 Apr 11;112(7):1339-1349
pubmed: 28402877
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
pubmed: 20057045
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14372-7
pubmed: 9405619
J Struct Biol. 2012 Oct;180(1):65-72
pubmed: 22634725
Genes Dev. 2012 Feb 1;26(3):203-34
pubmed: 22302935
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
pubmed: 16369096
FEBS J. 2012 Feb;279(4):563-71
pubmed: 22151054
J Fluoresc. 2011 May;21(3):873-81
pubmed: 20066479
Sci Rep. 2019 Oct 18;9(1):15007
pubmed: 31628352
J Cell Sci. 2016 Jun 15;129(12):2472
pubmed: 27307493
Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9811-E9820
pubmed: 29087344
Annu Rev Pharmacol Toxicol. 2000;40:617-47
pubmed: 10836149
J Mol Biol. 2009 Mar 6;386(4):913-9
pubmed: 19244612
Oncotarget. 2013 Oct;4(10):1550-1
pubmed: 24077698
FASEB J. 2015 Oct;29(10):4133-44
pubmed: 26103986
Biochim Biophys Acta. 2010 Oct-Dec;1799(10-12):775-87
pubmed: 20493977
Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):33-9
pubmed: 18094465
Biochim Biophys Acta Gen Subj. 2018 Jul;1862(7):1612-1625
pubmed: 29649512
Nature. 2019 Nov;575(7783):545-550
pubmed: 31581174
Trends Biochem Sci. 2005 Jan;30(1):43-52
pubmed: 15653325
Biophys Chem. 1988 Dec;32(2-3):173-85
pubmed: 3251567
Biochemistry. 2009 Oct 13;48(40):9456-65
pubmed: 19719323
J Biol Chem. 1999 May 21;274(21):14823-30
pubmed: 10329681
Mol Cell Proteomics. 2011 Oct;10(10):M110.005751
pubmed: 21725060
Nat Protoc. 2011 Jun;6(6):761-71
pubmed: 21637196
Biotechnol Prog. 2004 Nov-Dec;20(6):1847-54
pubmed: 15575721
PLoS One. 2012;7(1):e29554
pubmed: 22279540
J Biol Chem. 2003 Apr 11;278(15):12722-8
pubmed: 12551948
Semin Cancer Biol. 2006 Jun;16(3):162-72
pubmed: 16678438
Biochem Biophys Res Commun. 2017 Nov 18;493(2):940-945
pubmed: 28943433
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Annu Rev Immunol. 1998;16:225-60
pubmed: 9597130
FEBS Lett. 2018 Apr;592(7):1211-1220
pubmed: 29473952
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):271-81
pubmed: 21460445
J Biol Chem. 2019 Aug 30;294(35):13106-13116
pubmed: 31308176
J Biol Chem. 1996 Aug 16;271(33):20029-34
pubmed: 8702721
Structure. 2017 Feb 7;25(2):305-316
pubmed: 28089448
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14
pubmed: 23793146
Cell. 1997 Dec 26;91(7):961-71
pubmed: 9428519
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):E1152-61
pubmed: 26888287
Biochem J. 2017 Mar 23;474(7):1273-1287
pubmed: 28202711
Bioinformatics. 2015 Jul 15;31(14):2276-83
pubmed: 25735772
J Biol Chem. 2001 Nov 30;276(48):45225-35
pubmed: 11571291
Mol Cell Biol. 1997 Sep;17(9):5386-99
pubmed: 9271416
J Biol Chem. 2007 Mar 30;282(13):9411-9
pubmed: 17255105
Annu Rev Immunol. 1996;14:649-83
pubmed: 8717528