Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint.
MAD
Cyclin B1
RZZ complex
kinetochore corona
spindle assembly checkpoint
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
17 06 2020
17 06 2020
Historique:
received:
08
08
2019
revised:
20
01
2020
accepted:
02
03
2020
pubmed:
24
3
2020
medline:
7
4
2021
entrez:
24
3
2020
Statut:
ppublish
Résumé
Cyclin B:CDK1 is the master kinase regulator of mitosis. We show here that, in addition to its kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help preserve genome stability. Cyclin B1 localises to an expanded region of the outer kinetochore, known as the corona, where it scaffolds the spindle assembly checkpoint (SAC) machinery by binding directly to MAD1. In vitro reconstitutions map the key binding interface to a few acidic residues in the N-terminal region of MAD1, and point mutations in this sequence abolish MAD1 corona localisation and weaken the SAC. Therefore, Cyclin B1 is the long-sought-after scaffold that links MAD1 to the corona, and this specific pool of MAD1 is needed to generate a robust SAC response. Robustness arises because Cyclin B1:MAD1 localisation loses dependence on MPS1 kinase after the corona has been established, ensuring that corona-localised MAD1 can still be phosphorylated when MPS1 activity is low. Therefore, this study explains how corona-MAD1 generates a robust SAC signal, and it reveals a scaffolding role for the key mitotic kinase, Cyclin B1:CDK1, which ultimately helps to inhibit its own degradation.
Identifiants
pubmed: 32202322
doi: 10.15252/embj.2019103180
pmc: PMC7298293
doi:
Substances chimiques
CCNB1 protein, human
0
Cell Cycle Proteins
0
Cyclin B1
0
MAD1L1 protein, human
0
CDC2 Protein Kinase
EC 2.7.11.22
CDK1 protein, human
EC 2.7.11.22
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e103180Subventions
Organisme : Cancer Research UK (CRUK)
ID : C47320
Organisme : Ninewells Cancer Campaign and the Leng Charitable Trust
Organisme : Max Planck Society
Organisme : European Research Council Advanced Investigator
ID : RECEPIANCE (proposal 669686)
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
Références
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7313-8
pubmed: 24711418
Cell Rep. 2020 Apr 28;31(4):107535
pubmed: 32348762
Am Psychol. 2005 Feb-Mar;60(2):170-80
pubmed: 15740449
Curr Biol. 2012 May 22;22(10):891-9
pubmed: 22521786
Elife. 2017 Jan 10;6:
pubmed: 28072388
PLoS Biol. 2019 Mar 27;17(3):e3000202
pubmed: 30917112
Cell. 2014 Feb 27;156(5):1017-31
pubmed: 24581499
EMBO J. 2009 Apr 8;28(7):902-14
pubmed: 19229290
J Cell Sci. 2019 Jun 17;132(12):
pubmed: 31138679
Curr Biol. 2018 Nov 5;28(21):3422-3429.e5
pubmed: 30415700
J Cell Biol. 2019 Apr 1;218(4):1108-1117
pubmed: 30674583
Dev Cell. 2017 Feb 27;40(4):405-420.e2
pubmed: 28216383
Nat Cell Biol. 2018 Jul;20(7):800-810
pubmed: 29915359
Dev Cell. 2015 Dec 7;35(5):600-613
pubmed: 26651294
J Struct Biol. 2007 Jan;157(1):38-46
pubmed: 16859925
Curr Biol. 2012 May 22;22(10):900-6
pubmed: 22521787
EMBO J. 2020 Jun 17;39(12):e103180
pubmed: 32202322
Biology (Basel). 2017 Jan 24;6(1):
pubmed: 28125021
Mol Biol Cell. 2008 Nov;19(11):4918-29
pubmed: 18799620
Science. 2015 Jun 12;348(6240):1264-7
pubmed: 26068855
Stat Med. 2009 Jan 30;28(2):205-20
pubmed: 18991332
J Cell Biol. 2015 Sep 14;210(6):899-916
pubmed: 26347137
Nat Cell Biol. 2001 Nov;3(11):1001-7
pubmed: 11715021
Open Biol. 2016 Feb;6(2):150236
pubmed: 26911624
Mol Cell. 2017 Nov 16;68(4):715-730.e5
pubmed: 29129638
Cell. 2004 Mar 5;116(5):683-98
pubmed: 15006351
Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5839-44
pubmed: 20167803
J Cell Biol. 2019 Apr 1;218(4):1182-1199
pubmed: 30674582
Nature. 2017 Feb 23;542(7642):498-502
pubmed: 28102834
Dev Cell. 2015 Feb 9;32(3):358-372
pubmed: 25669885
EMBO J. 2019 Nov 15;38(22):e102423
pubmed: 31642077
Mol Cell. 2015 Mar 5;57(5):824-835
pubmed: 25661489
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9
pubmed: 27114506
J Cell Biol. 2005 Apr 11;169(1):49-60
pubmed: 15824131
Open Biol. 2015 Nov;5(11):
pubmed: 26581576
Curr Biol. 2005 May 10;15(9):856-61
pubmed: 15886105
EMBO J. 2005 Apr 20;24(8):1621-33
pubmed: 15933723
Nat Commun. 2011;2:316
pubmed: 21587233
Nat Cell Biol. 2013 Nov;15(11):1370-7
pubmed: 24096243
Science. 2016 Jun 3;352(6290):1180-1
pubmed: 27257246
J Biol Chem. 2018 Jan 12;293(2):484-496
pubmed: 29162720
Nat Cell Biol. 2012 Jun 03;14(7):746-52
pubmed: 22660415
J Cell Biol. 2013 Dec 23;203(6):943-55
pubmed: 24344183
J Cell Biol. 2010 Jul 12;190(1):25-34
pubmed: 20624899
Front Cell Dev Biol. 2018 Jun 19;6:62
pubmed: 29971233
Science. 2015 Jun 12;348(6240):1260-4
pubmed: 26068854
J Cell Biol. 2010 Oct 18;191(2):281-90
pubmed: 20937696
Curr Biol. 2016 Oct 10;26(19):2642-2650
pubmed: 27618268
Elife. 2016 Dec 24;5:
pubmed: 28012276
EMBO J. 2019 Apr 1;38(7):
pubmed: 30782962
Nat Cell Biol. 2011 Apr;13(4):475-82
pubmed: 21394085
Prog Mol Subcell Biol. 2017;56:429-455
pubmed: 28840248
Cancer Res. 2008 Nov 15;68(22):9194-201
pubmed: 19010891
J Cell Biol. 2014 Mar 3;204(5):647-57
pubmed: 24567362
Mol Biol Cell. 2007 Dec;18(12):4847-58
pubmed: 17881737
Oncotarget. 2015 Aug 28;6(25):20921-32
pubmed: 26320186
J Cell Biol. 2008 Jun 16;181(6):893-901
pubmed: 18541701
Nat Commun. 2017 Jun 12;8:15822
pubmed: 28604727
J Cell Biol. 1995 Aug;130(4):941-8
pubmed: 7642709
Cell Res. 2008 Feb;18(2):268-80
pubmed: 18195732
Front Cell Dev Biol. 2018 May 07;6:51
pubmed: 29868582
Curr Biol. 2018 Nov 5;28(21):3408-3421.e8
pubmed: 30415699
J Cell Sci. 2015 Aug 15;128(16):2975-82
pubmed: 26148513
Psychol Sci. 2014 Jan;25(1):7-29
pubmed: 24220629
Genes Dev. 2014 Jan 15;28(2):140-52
pubmed: 24402315
J Cell Biol. 2020 Jun 1;219(6):
pubmed: 32236513
Nat Cell Biol. 2014 Dec;16(12):1257-64
pubmed: 25402682
J Cell Biol. 2001 Dec 24;155(7):1159-72
pubmed: 11756470
J Cell Biol. 2009 Sep 7;186(5):675-84
pubmed: 19720871
Curr Opin Cell Biol. 2016 Apr;39:101-8
pubmed: 26947988
Cell Rep. 2019 Aug 20;28(8):2206-2219.e8
pubmed: 31433993