PP2A phosphatase is required for dendrite pruning via actin regulation in Drosophila.
PP2A
actin
dendrite
phosphatase
pruning
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
06 05 2020
06 05 2020
Historique:
received:
15
07
2019
revised:
27
02
2020
accepted:
04
03
2020
pubmed:
25
3
2020
medline:
28
4
2021
entrez:
25
3
2020
Statut:
ppublish
Résumé
Large-scale pruning, the developmentally regulated degeneration of axons or dendrites, is an important specificity mechanism during neuronal circuit formation. The peripheral sensory class IV dendritic arborization (c4da) neurons of Drosophila larvae specifically prune their dendrites at the onset of metamorphosis in an ecdysone-dependent manner. Dendrite pruning requires local cytoskeleton remodeling, and the actin-severing enzyme Mical is an important ecdysone target. In a screen for pruning factors, we identified the protein phosphatase 2 A (PP2A). PP2A interacts genetically with the actin-severing enzymes Mical and cofilin as well as other actin regulators during pruning. Moreover, Drosophila cofilin undergoes a change in localization at the onset of metamorphosis indicative of a change in actin dynamics. This change is abolished both upon loss of Mical and PP2A. We conclude that PP2A regulates actin dynamics during dendrite pruning.
Identifiants
pubmed: 32207238
doi: 10.15252/embr.201948870
pmc: PMC7202059
doi:
Substances chimiques
Actins
0
Drosophila Proteins
0
Protein Phosphatase 2
EC 3.1.3.16
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48870Subventions
Organisme : Excellence Cluster Cells-in-Motion, DFG
ID : EXC1003
Pays : International
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
Références
J Cell Biol. 2018 Oct 1;217(10):3731-3746
pubmed: 30042190
EMBO J. 2017 Jul 3;36(13):1981-1991
pubmed: 28554895
Nat Neurosci. 2009 Dec;12(12):1497-505
pubmed: 19881505
EMBO Rep. 2020 May 6;21(5):e48870
pubmed: 32207238
Genes Dev. 2011 Sep 15;25(18):1982-96
pubmed: 21937715
Nat Neurosci. 2006 Oct;9(10):1234-6
pubmed: 16980964
Sci Rep. 2016 Mar 15;6:23102
pubmed: 26976084
Development. 2019 Apr 4;146(7):
pubmed: 30910826
Neuron. 1999 Mar;22(3):451-61
pubmed: 10197526
Neuron. 2012 Dec 20;76(6):1091-107
pubmed: 23259946
Nature. 2010 Feb 11;463(7282):823-7
pubmed: 20148037
Dev Biol. 2019 Jul 1;451(1):68-78
pubmed: 30871987
Cell Rep. 2018 Aug 28;24(9):2287-2299.e4
pubmed: 30157424
Genesis. 2002 Sep-Oct;34(1-2):107-10
pubmed: 12324961
Nature. 2007 Jul 12;448(7150):151-6
pubmed: 17625558
Nat Cell Biol. 2015 Jan;17(1):68-80
pubmed: 25531779
Cell Motil Cytoskeleton. 2009 Aug;66(8):635-49
pubmed: 19479823
Neuron. 2014 Feb 5;81(3):544-560
pubmed: 24412417
PLoS Genet. 2020 Feb 14;16(2):e1008626
pubmed: 32059017
Nature. 1998 Jun 25;393(6687):805-9
pubmed: 9655397
J Neurosci. 2012 Nov 28;32(48):17086-96
pubmed: 23197702
Annu Rev Cell Dev Biol. 2015;31:779-805
pubmed: 26436703
Development. 2002 Jun;129(12):2867-78
pubmed: 12050135
PLoS Biol. 2013 Sep;11(9):e1001657
pubmed: 24068890
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Nature. 1998 Jun 25;393(6687):809-12
pubmed: 9655398
Cell. 2002 Jan 25;108(2):233-46
pubmed: 11832213
Cell. 1995 Feb 24;80(4):553-62
pubmed: 7867063
Dev Cell. 2014 Aug 25;30(4):463-78
pubmed: 25158855
Cell Mol Life Sci. 2015 Jan;72(1):101-19
pubmed: 25213356
Sci Signal. 2017 Oct 31;10(503):
pubmed: 29089450
Oncogene. 1999 Jan 7;18(1):219-32
pubmed: 9926937
Genetics. 2017 Dec;207(4):1401-1421
pubmed: 29025914
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9715-20
pubmed: 18621688
Science. 2011 Dec 23;334(6063):1710-3
pubmed: 22116028
Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15230-5
pubmed: 16210248
Cell. 2002 Jun 28;109(7):887-900
pubmed: 12110185
Nat Cell Biol. 2016 Aug;18(8):876-85
pubmed: 27454820
Neuron. 1996 Dec;17(6):1201-7
pubmed: 8982166
Dev Cell. 2009 Jun;16(6):901-8
pubmed: 19531360
Development. 2018 May 11;145(9):
pubmed: 29712642
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7
pubmed: 17360644
J Neurosci. 2012 Aug 22;32(34):11631-42
pubmed: 22915107
Elife. 2020 Feb 13;9:
pubmed: 32053108
Nat Commun. 2015 Mar 12;6:6515
pubmed: 25761586
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6363-8
pubmed: 19329489
PLoS One. 2017 Oct 4;12(10):e0186037
pubmed: 28977036
Neuron. 2004 Dec 2;44(5):779-93
pubmed: 15572110
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7331-6
pubmed: 24799714
Dev Dyn. 2010 Oct;239(10):2685-94
pubmed: 20803583
Trends Cell Biol. 2019 Apr;29(4):291-297
pubmed: 30683460
Nat Methods. 2008 Jul;5(7):605-7
pubmed: 18536722
EMBO Rep. 2020 May 6;21(5):e48843
pubmed: 32187821
Development. 2007 Jan;134(1):55-64
pubmed: 17164414
Development. 2002 Jul;129(14):3493-503
pubmed: 12091318
Development. 2005 Aug;132(16):3631-42
pubmed: 16033801