EV-Ident: Identifying Tumor-Specific Extracellular Vesicles by Size Fractionation and Single-Vesicle Analysis.

Antigens, Surface / blood Biomarkers, Tumor / blood Breast Neoplasms / blood Extracellular Vesicles / chemistry Female Glutamate Carboxypeptidase II / blood Humans Male Particle Size Prostatic Neoplasms / blood Receptor, ErbB-2 / blood Surface Properties

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
21 04 2020
Historique:
pubmed: 26 3 2020
medline: 10 2 2021
entrez: 26 3 2020
Statut: ppublish

Résumé

Tumor-derived extracellular vesicles (EVs) have emerged as a promising source of circulating biomarkers for liquid biopsies. However, understanding the heterogeneous physical and biochemical properties of EVs originating from multiple complex biogenesis pathways remains a major challenge. Here, we introduce EV-Ident for preparation of subpopulations of EVs in three different size fractions: large EVs (EV

Identifiants

DOI: 10.1021/acs.analchem.0c00285 PMID: 32207920
pubmed: 32207920
doi: 10.1021/acs.analchem.0c00285
doi:

Substances chimiques

Antigens, Surface 0
Biomarkers, Tumor 0
ERBB2 protein, human EC 2.7.10.1
Receptor, ErbB-2 EC 2.7.10.1
FOLH1 protein, human EC 3.4.17.21
Glutamate Carboxypeptidase II EC 3.4.17.21

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6010-6018

Auteurs

Dongyoung Kim (D)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

Hyun-Kyung Woo (HK)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.

Chaeeun Lee (C)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.

Yoohong Min (Y)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

Sumit Kumar (S)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

Vijaya Sunkara (V)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

Hwi-Gyeong Jo (HG)

Department of Biomedical Science, Asan Medical Center, Seoul 05505, Republic of Korea.

Young Joo Lee (YJ)

Division of Breast Surgery, Department of Surgery, University of Ulsan College Medicine, Asan Medical Center, Seoul 05505, Republic of Korea.

Jisun Kim (J)

Division of Breast Surgery, Department of Surgery, University of Ulsan College Medicine, Asan Medical Center, Seoul 05505, Republic of Korea.

Hong Koo Ha (HK)

Department of Urology, Pusan National University Hospital, College of Medicine, Pusan National University, Busan 49241, Republic of Korea.
Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Republic of Korea.

Yoon-Kyoung Cho (YK)

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
ORCID: 0000-0001-6423-1834

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Maladies de la peau et du tissu conjonctif › Maladies de la peau › Maladies du sein › Tumeurs du sein › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Metalloproteases › Metalloexopeptidases › Glutamate carboxypeptidase II › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Phénomènes chimiques › Taille de particule › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Maladies urogénitales › Tumeurs de l'appareil urogénital › Tumeurs de l'appareil génital mâle › Tumeurs de la prostate › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Récepteur ErbB-2 › EV-Ident: Identifying Tumor-Specific...
questionsmedicales.fr › Phénomènes chimiques › Propriétés de surface › EV-Ident: Identifying Tumor-Specific...