Upconversion Nonlinear Structured Illumination Microscopy.

NIR SIM deep tissue nonlinear super-resolution upconversion nanoparticles

Journal

Nano letters
ISSN: 1530-6992
Titre abrégé: Nano Lett
Pays: United States
ID NLM: 101088070

Informations de publication

Date de publication:
08 07 2020
Historique:
pubmed: 27 3 2020
medline: 27 3 2020
entrez: 27 3 2020
Statut: ppublish

Résumé

Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong extinction of light by biological tissues, which restricts the imaging depth and degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm upconverted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in the Fourier domain. These strategies lead to a new modality in microscopy with a resolution below 131 nm, 1/7th of the excitation wavelength, and an imaging rate of 1 Hz.

Identifiants

DOI: 10.1021/acs.nanolett.0c00448 PMID: 32208705
pubmed: 32208705
doi: 10.1021/acs.nanolett.0c00448
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4775-4781

Auteurs

Baolei Liu (B)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
School of Mathematical and Physical Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Sydney, NSW 2007, Australia.

Chaohao Chen (C)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

Xiangjun Di (X)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

Jiayan Liao (J)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
ORCID: 0000-0003-0616-4762

Shihui Wen (S)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

Qian Peter Su (QP)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

Xuchen Shan (X)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

Zai-Quan Xu (ZQ)

School of Mathematical and Physical Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Sydney, NSW 2007, Australia.

Lining Arnold Ju (LA)

School of Biomedical Engineering, Faculty of Engineering and Charles Perkins Centre, The University of Sydney, Camperdown, NSW 2006, Australia.
Heart Research Institute, Newtown, NSW 2042, Australia.

Chao Mi (C)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
ORCID: 0000-0003-0895-9948

Fan Wang (F)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
ORCID: 0000-0001-7403-3305

Dayong Jin (D)

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Classifications MeSH