Integrative Analysis of Breast Cancer Cells Reveals an Epithelial-Mesenchymal Transition Role in Adaptation to Acidic Microenvironment.
EMT
S100 family proteins
acid adaptation
breast cancer
tumor microenvironment
Journal
Frontiers in oncology
ISSN: 2234-943X
Titre abrégé: Front Oncol
Pays: Switzerland
ID NLM: 101568867
Informations de publication
Date de publication:
2020
2020
Historique:
received:
03
11
2019
accepted:
20
02
2020
entrez:
27
3
2020
pubmed:
27
3
2020
medline:
27
3
2020
Statut:
epublish
Résumé
Early ducts of breast tumors are unequivocally acidic. High rates of glycolysis combined with poor perfusion lead to a congestion of acidic metabolites in the tumor microenvironment, and pre-malignant cells must adapt to this acidosis to thrive. Adaptation to acidosis selects cancer cells that can thrive in harsh conditions and are capable of outgrowing the normal or non-adapted neighbors. This selection is usually accompanied by phenotypic change. Epithelial mesenchymal transition (EMT) is one of the most important switches correlated to malignant tumor cell phenotype and has been shown to be induced by tumor acidosis. New evidence shows that the EMT switch is not a binary system and occurs on a spectrum of transition states. During confirmation of the EMT phenotype, our results demonstrated a partial EMT phenotype in our acid-adapted cell population. Using RNA sequencing and network analysis we found 10 dysregulated network motifs in acid-adapted breast cancer cells playing a role in EMT. Our further integrative analysis of RNA sequencing and SILAC proteomics resulted in recognition of S100B and S100A6 proteins at both the RNA and protein level. Higher expression of S100B and S100A6 was validated
Identifiants
pubmed: 32211331
doi: 10.3389/fonc.2020.00304
pmc: PMC7076123
doi:
Types de publication
Journal Article
Langues
eng
Pagination
304Subventions
Organisme : NCI NIH HHS
ID : P30 CA076292
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA077575
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA193489
Pays : United States
Informations de copyright
Copyright © 2020 Sadeghi, Ordway, Rafiei, Borad, Fang, Koomen, Zhang, Yoder, Johnson and Damaghi.
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Bioinformatics. 2008 Jan 15;24(2):282-4
pubmed: 18006545
Nat Commun. 2015 Dec 10;6:8752
pubmed: 26658462
Trends Endocrinol Metab. 2016 Oct;27(10):681-695
pubmed: 27372267
Cancer Cell. 2018 Sep 10;34(3):427-438.e6
pubmed: 30205045
Nat Commun. 2019 Apr 16;10(1):1787
pubmed: 30992437
F1000Res. 2018 Nov 19;7:
pubmed: 30519451
Nat Protoc. 2011 Feb;6(2):147-57
pubmed: 21293456
Nat Methods. 2009 Sep;6(9):647-9
pubmed: 19668204
Nucleic Acids Res. 2003 Jan 1;31(1):258-61
pubmed: 12519996
Cancer Res. 2013 Mar 1;73(5):1524-35
pubmed: 23288510
PLoS One. 2017 Sep 18;12(9):e0185085
pubmed: 28922380
Cell Adh Migr. 2015;9(3):233-46
pubmed: 25482613
Nat Commun. 2019 Dec 6;10(1):5587
pubmed: 31811131
Bull Math Biol. 2018 May;80(5):954-970
pubmed: 28508297
Cell Cycle. 2017 Oct 2;16(19):1739-1743
pubmed: 27635863
Cancer Res. 2012 Aug 15;72(16):3938-47
pubmed: 22719070
Nat Rev Genet. 2007 Jun;8(6):450-61
pubmed: 17510665
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
Comput Biol Chem. 2012 Apr;37:17-23
pubmed: 22430954
Oncol Rep. 2019 Oct;42(4):1307-1318
pubmed: 31364741
N Engl J Med. 2012 Mar 8;366(10):883-892
pubmed: 22397650
Cancer Res. 2017 May 1;77(9):2242-2254
pubmed: 28249898
Int J Cancer. 2017 Mar 15;140(6):1331-1345
pubmed: 27888521
J Leukoc Biol. 2001 Apr;69(4):522-30
pubmed: 11310837
Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4009-14
pubmed: 23412337
World J Stem Cells. 2015 Jan 26;7(1):27-36
pubmed: 25621103
F1000Res. 2015 Aug 05;4:479
pubmed: 26594341
Cancer Res. 2016 Mar 15;76(6):1381-90
pubmed: 26719539
J Biol Chem. 2002 Mar 29;277(13):11368-74
pubmed: 11741977
Wiley Interdiscip Rev Syst Biol Med. 2011 Sep-Oct;3(5):544-61
pubmed: 21197660
Dev Cell. 2018 Jun 18;45(6):681-695.e4
pubmed: 29920274
Carcinogenesis. 2010 Jan;31(1):2-8
pubmed: 19861649
Nat Commun. 2017 Aug 4;8(1):198
pubmed: 28775339
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Nat Rev Genet. 2009 Jan;10(1):57-63
pubmed: 19015660
Cell Stem Cell. 2019 Jan 3;24(1):65-78
pubmed: 30554963
J Cancer. 2015 Jan 01;6(1):54-65
pubmed: 25553089
Clin Chem. 2013 Jan;59(1):168-79
pubmed: 23220226
Oncogene. 2010 Apr 22;29(16):2346-56
pubmed: 20118985
Integr Biol (Camb). 2015 Jan;7(1):14-23
pubmed: 25359461
Nat Commun. 2018 Jul 31;9(1):2997
pubmed: 30065243
Cancer Res. 2017 Dec 1;77(23):6538-6550
pubmed: 28947419
Nat Rev Cancer. 2008 Jan;8(1):56-61
pubmed: 18059462
PLoS One. 2016 Dec 22;11(12):e0168760
pubmed: 28005952
Front Oncol. 2018 Apr 19;8:117
pubmed: 29725585
J Nucl Med. 2008 Jun;49 Suppl 2:24S-42S
pubmed: 18523064
Pharmacol Ther. 2019 Feb;194:161-184
pubmed: 30268772
BMC Med. 2013 Jun 18;11:151
pubmed: 23800221
N Engl J Med. 2013 Jul 11;369(2):122-33
pubmed: 23724867
Cancer Inform. 2014 Oct 16;13(Suppl 5):37-47
pubmed: 25392692
BMC Syst Biol. 2017 Jun 15;11(1):61
pubmed: 28619054
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Methods Mol Biol. 2019;1928:441-468
pubmed: 30725469
Nat Protoc. 2009;4(5):698-705
pubmed: 19373234
Front Physiol. 2013 Dec 17;4:370
pubmed: 24381558
Cancer Cell Int. 2014 Nov 30;14(1):129
pubmed: 25493076
Cancer Res. 2000 Dec 15;60(24):7075-83
pubmed: 11156414
Methods Mol Biol. 2016;1344:147-81
pubmed: 26520123
Cancer Metastasis Rev. 2007 Jun;26(2):311-7
pubmed: 17404691
Nature. 2012 Apr 18;486(7403):346-52
pubmed: 22522925
Oncogene. 2013 Nov 7;32(45):5253-60
pubmed: 23416985