Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 03 2020
25 03 2020
Historique:
received:
16
08
2019
accepted:
04
03
2020
entrez:
28
3
2020
pubmed:
28
3
2020
medline:
15
7
2020
Statut:
epublish
Résumé
Understanding the nano-architecture of protein machines in diverse subcellular compartments remains a challenge despite rapid progress in super-resolution microscopy. While single-molecule localization microscopy techniques allow the visualization and identification of cellular structures with near-molecular resolution, multiplex-labeling of tens of target proteins within the same sample has not yet been achieved routinely. However, single sample multiplexing is essential to detect patterns that threaten to get lost in multi-sample averaging. Here, we report maS
Identifiants
pubmed: 32214101
doi: 10.1038/s41467-020-15362-1
pii: 10.1038/s41467-020-15362-1
pmc: PMC7096454
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1552Références
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