The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord.
Angiogenesis
Axonal growth
Composite
Hydrogel
Macrophage polarization
Nanofiber
Neurogenesis
Spinal cord injury
Journal
Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
10
03
2020
accepted:
15
03
2020
pubmed:
29
3
2020
medline:
15
5
2021
entrez:
29
3
2020
Statut:
ppublish
Résumé
An injury to the spinal cord causes long-lasting loss of nervous tissue because endogenous nervous tissue repair and regeneration at the site of injury is limited. We engineered an injectable nanofiber-hydrogel composite (NHC) with interfacial bonding to provide mechanical strength and porosity and examined its effect on repair and neural tissue regeneration in an adult rat model of spinal cord contusion. At 28 days after treatment with NHC, the width of the contused spinal cord segment was 2-fold larger than in controls. With NHC treatment, tissue in the injury had a 2-fold higher M2/M1 macrophage ratio, 5-fold higher blood vessel density, 2.6-fold higher immature neuron presence, 2.4-fold higher axon density, and a similar glial scar presence compared with controls. Spared nervous tissue volume in the contused segment and hind limb function was similar between groups. Our findings indicated that NHC provided mechanical support to the contused spinal cord and supported pro-regenerative macrophage polarization, angiogenesis, axon growth, and neurogenesis in the injured tissue without any exogenous factors or cells. These results motivate further optimization of the NHC and delivery protocol to fully translate the potential of the unique properties of the NHC for treating spinal cord injury.
Identifiants
pubmed: 32217415
pii: S0142-9612(20)30224-6
doi: 10.1016/j.biomaterials.2020.119978
pmc: PMC8787820
mid: NIHMS1579419
pii:
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
119978Subventions
Organisme : RRD VA
ID : I01 RX001807
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS085714
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS101298
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Neurosci Lett. 2011 Jan 25;488(3):299-304
pubmed: 21111028
J Control Release. 2012 Apr 30;159(2):271-80
pubmed: 22227024
Cell Mol Life Sci. 2015 Apr;72(7):1303-16
pubmed: 25504084
J Neurotrauma. 1995 Feb;12(1):1-21
pubmed: 7783230
J Neurosci. 2008 Apr 2;28(14):3814-23
pubmed: 18385339
Biomaterials. 2006 Apr;27(11):2370-9
pubmed: 16325904
Spine (Phila Pa 1976). 2001 Dec 15;26(24 Suppl):S13-22
pubmed: 11805602
Brain Res. 2015 Sep 4;1619:1-11
pubmed: 25578260
ACS Appl Mater Interfaces. 2017 Mar 1;9(8):6725-6745
pubmed: 28181797
J Neurosci. 2009 Oct 28;29(43):13435-44
pubmed: 19864556
J Neurosurg Spine. 2004 Oct;1(3):322-9
pubmed: 15478371
Cell Tissue Res. 2016 Apr;364(1):17-28
pubmed: 26463048
J Biomed Mater Res A. 2010 Jun 1;93(3):1091-9
pubmed: 19768787
J Tissue Eng Regen Med. 2018 Mar;12(3):e1725-e1736
pubmed: 27863083
Biomaterials. 2016 Apr;84:157-166
pubmed: 26828681
J Cell Sci. 2003 May 15;116(Pt 10):1881-92
pubmed: 12692189
J Neurosci Res. 2014 Mar;92(3):307-17
pubmed: 24375695
Biomaterials. 2010 Oct;31(30):7631-9
pubmed: 20656347
J Neuropathol Exp Neurol. 2002 Jul;61(7):623-33
pubmed: 12125741
J Neurosurg. 2001 Oct;95(2 Suppl):221-4
pubmed: 11599840
JAMA Neurol. 2015 Feb;72(2):235-7
pubmed: 25531583
Biotechnol Bioeng. 2003 Jun 5;82(5):578-89
pubmed: 12652481
Exp Neurol. 1992 Apr;116(1):40-51
pubmed: 1559563
Exp Neurol. 2002 Jan;173(1):63-76
pubmed: 11771939
J Neurotrauma. 1992 Mar;9 Suppl 1:S83-91
pubmed: 1588634
Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17253-8
pubmed: 24101477
J Neurosci Res. 2008 Feb 15;86(3):581-92
pubmed: 17918743
J Tissue Eng Regen Med. 2015 Nov;9(11):1298-309
pubmed: 23401421
J Neural Eng. 2011 Aug;8(4):046033
pubmed: 21753237
J Neurotrauma. 2010 Jan;27(1):1-19
pubmed: 19698073
ACS Biomater Sci Eng. 2015 Jul 13;1(7):481-493
pubmed: 26280020
Nat Commun. 2017 Sep 14;8(1):533
pubmed: 28912446
Neurosci Lett. 2012 Jun 25;519(2):115-21
pubmed: 22343313
Stem Cells Transl Med. 2014 May;3(5):662-70
pubmed: 24692587
Biomaterials. 2009 Mar;30(8):1482-91
pubmed: 19121538
J Neurotrauma. 2014 Aug 15;31(16):1431-8
pubmed: 24447305
PLoS One. 2011;6(5):e19782
pubmed: 21611127
Biomaterials. 2014 Feb;35(6):1924-31
pubmed: 24331711
J Spinal Cord Med. 2012 Jan;35(1):68-9
pubmed: 22330194
Sci Transl Med. 2019 May 1;11(490):
pubmed: 31043572
J Biomed Mater Res B Appl Biomater. 2010 Oct;95(1):110-7
pubmed: 20725955
Exp Neurol. 2014 Oct;260:44-9
pubmed: 23376590
Biomaterials. 2016 Jan;74:89-98
pubmed: 26454047
Biomaterials. 2014 Jul;35(22):5700-10
pubmed: 24746961
Interface Focus. 2011 Oct 6;1(5):725-33
pubmed: 23050077
Theranostics. 2018 Aug 07;8(16):4429-4446
pubmed: 30214630
Biomed Mater. 2018 Apr 25;13(4):044105
pubmed: 29359704
Science. 1985 Jun 14;228(4705):1324-6
pubmed: 2408340
J Neurotrauma. 2004 Jun;21(6):789-804
pubmed: 15253805
ASN Neuro. 2013 May 30;5(2):149-66
pubmed: 23614684
ACS Nano. 2015 Sep 22;9(9):9167-79
pubmed: 26280983
Biomaterials. 2017 Sep;138:91-107
pubmed: 28554011
Acta Biomater. 2015 Jan;11:204-11
pubmed: 25266504
Neurotherapeutics. 2018 Jul;15(3):578-587
pubmed: 29728851
Int J Nanomedicine. 2018 May 17;13:2883-2895
pubmed: 29844671
Biomaterials. 2018 Dec;185:284-300
pubmed: 30265898
J Mater Chem B. 2017 Jun 7;5(21):3870-3878
pubmed: 28775848
J Neurotrauma. 2006 Mar-Apr;23(3-4):264-80
pubmed: 16629615
Cells Tissues Organs. 2013;197(1):55-63
pubmed: 22796886
Biomaterials. 2012 Jun;33(18):4555-64
pubmed: 22459192
Biomaterials. 2013 May;34(15):3775-83
pubmed: 23465486