Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer.
Brown adipose tissue
Energy metabolism
Mitochondrial function
Seahorse analyzer
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
29
3
2020
pubmed:
29
3
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Brown adipocytes are a cell type with high mitochondrial content and bioenergetic capacity. A critical means to measure mitochondrial function, macromolecule fuel usage, and other important phenotypes is with a bioenergetic analyzer. Here, we describe how to isolate, culture, and differentiate brown preadipocytes into mature adipocytes. We also explain how to perform a mitochondrial (mito) stress test, using the bioenergetic analyzer. The mito stress test is able to give researchers a plethora of insights into mitochondrial function, including basal respiration, proton leak, ATP production, maximal respiration, and reserve capacity, making it a powerful tool for analyzing brown adipocytes.
Identifiants
pubmed: 32219757
doi: 10.1007/978-1-0716-0471-7_20
pmc: PMC8075131
mid: NIHMS1691350
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-296Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK036836
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK077097
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK102898
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007260
Pays : United States
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