Generating primed pluripotent epiblast stem cells: A methodology chapter.
ESC
EpiLC
EpiSC
X-chromosome inactivation
Journal
Current topics in developmental biology
ISSN: 1557-8933
Titre abrégé: Curr Top Dev Biol
Pays: United States
ID NLM: 0163114
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
30
3
2020
pubmed:
30
3
2020
medline:
4
6
2021
Statut:
ppublish
Résumé
At least two distinct pluripotent states, referred to as naïve and primed, define the early mammalian embryo. In the mouse, the pluripotent epiblast cells in the pre/peri-implantation embryo are the source of naïve embryonic stem cells (ESCs). After the embryo implants, the epiblast lineage generates a restricted or primed population of stem cells, referred to as epiblast stem cells (EpiSCs). ESCs can be cultured in EpiSC media to generate epiblast-like cells (EpiLCs). The differentiation of naive ESCs into primed EpiLCs permits insights into the development and differentiation of the pluripotent epiblast lineage. This chapter describes the generation and characterization of EpiSCs as well as EpiLCs.
Identifiants
pubmed: 32220296
pii: S0070-2153(20)30026-0
doi: 10.1016/bs.ctdb.2020.01.005
pmc: PMC7311189
mid: NIHMS1600117
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
139-174Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM124571
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD095463
Pays : United States
Informations de copyright
© 2020 Elsevier Inc. All rights reserved.
Références
Cell Rep. 2015 May 26;11(8):1251-65
pubmed: 25981039
Nature. 2016 Sep 1;537(7618):57-62
pubmed: 27556940
J Embryol Exp Morphol. 1985 Dec;90:379-88
pubmed: 3834036
Nature. 1981 Jul 9;292(5819):154-6
pubmed: 7242681
Cell. 2011 Aug 19;146(4):519-32
pubmed: 21820164
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5709-12
pubmed: 9159137
Nature. 1959 Oct 24;184:1286-7
pubmed: 13836947
Nature. 1979 Sep 27;281(5729):311-3
pubmed: 551278
J Embryol Exp Morphol. 1967 Aug;18(1):155-80
pubmed: 6048976
Cell Stem Cell. 2014 Oct 2;15(4):416-430
pubmed: 25280218
Development. 2009 Apr;136(7):1063-9
pubmed: 19224983
J Embryol Exp Morphol. 1976 Oct;36(2):283-90
pubmed: 1033982
J Embryol Exp Morphol. 1983 Dec;78:1-22
pubmed: 6198418
Nature. 2007 Jul 12;448(7150):191-5
pubmed: 17597762
Science. 2004 Jan 30;303(5658):666-9
pubmed: 14752160
Nature. 1984 May 17-23;309(5965):255-6
pubmed: 6717601
Nature. 2007 Jul 12;448(7150):196-9
pubmed: 17597760
J Embryol Exp Morphol. 1983 Apr;74:207-20
pubmed: 6886595
Nature. 2009 Oct 29;461(7268):1292-5
pubmed: 19816418
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8
pubmed: 6950406
Nature. 1971 Jun 11;231(5302):385-6
pubmed: 4931003