High-Throughput Sequencing for Trypanosome Transcriptome Characterization.
Gene expression
Gene regulation
Mouse; Trypanosoma
RNASeq
Rat
Rodent
Transcriptome
rRNA depletion
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
30
3
2020
pubmed:
30
3
2020
medline:
2
2
2021
Statut:
ppublish
Résumé
High-throughput sequencing of cDNA (RNASeq) is now the method of choice for analysis of transcriptomes. This chapter details important considerations in the design of RNASeq experiments for kinetoplastids grown in culture or experimental animals. It contains protocols for obtaining parasites from rodents, and for removal of rRNA from total RNA. In addition, custom pipelines for sequence alignment, and for data analysis and visualization, are described.
Identifiants
pubmed: 32221915
doi: 10.1007/978-1-0716-0294-2_6
doi:
Substances chimiques
RNA, Messenger
0
RNA, Protozoan
0
RNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM