Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry.

Chromatography, Liquid Disaccharides / metabolism Extracellular Matrix / metabolism Glycosaminoglycans / metabolism Human Umbilical Vein Endothelial Cells / metabolism Humans Mesenchymal Stem Cells / metabolism Tandem Mass Spectrometry / methods
Compositional analysis Decellularized cell-derived extracellular matrices Disaccharides Glycosaminoglycans Liquid chromatography-tandem mass spectrometrty

Journal

Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334

Informations de publication

Date de publication:
2020
Historique:
entrez: 31 3 2020
pubmed: 31 3 2020
medline: 29 12 2020
Statut: ppublish

Résumé

Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, without the limitations of availability and potential pathogen transmission associated with tissue-derived extracellular matrix (ECM) scaffolds. Glycosaminoglycans (GAGs) are important components of ECM with a crucial role in the maintenance of the mechanical properties of the tissue and as signaling regulators of several cellular processes, such as cell adhesion, growth and differentiation. However, despite their relevance to the field of TE, little information is available on the GAG composition of cell-derived ECM, mainly due to the lack of appropriate quantitative tools to determine different GAG and disaccharide subtypes in complex biological samples. In this chapter, we describe a highly sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to characterize decellularized cell-derived ECM generated in vitro in terms of their GAG and disaccharide composition.

Identifiants

DOI: 10.1016/bs.mcb.2019.11.017 PMID: 32222228
pubmed: 32222228
pii: S0091-679X(19)30147-5
doi: 10.1016/bs.mcb.2019.11.017
pii:
doi:

Substances chimiques

Disaccharides 0
Glycosaminoglycans 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

85-106

Informations de copyright

© 2020 Elsevier Inc. All rights reserved.

Auteurs

João C Silva (JC)

Department of Bioengineering and iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Department of Chemistry and Chemical Biology, Biological Sciences and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States.

Marta S Carvalho (MS)

Department of Bioengineering and iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States.

Ke Xia (K)

Department of Chemistry and Chemical Biology, Biological Sciences and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States.

Joaquim M S Cabral (JMS)

Department of Bioengineering and iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Claúdia L da Silva (CL)

Department of Bioengineering and iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Frederico C Ferreira (FC)

Department of Bioengineering and iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Deepak Vashishth (D)

Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States.

Robert J Linhardt (RJ)

Department of Chemistry and Chemical Biology, Biological Sciences and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States; Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States. Electronic address: linhar@rpi.edu.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Glucides › Polyosides › Glycosaminoglycanes › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Cellules endothéliales de la veine ombilicale humaine › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Glycosaminoglycan disaccharide compositional...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Glycosaminoglycan disaccharide compositional...