Increased transcription of transglutaminase 1 mediates neuronal death in in vitro models of neuronal stress and Aβ1-42-mediated toxicity.


Journal

Neurobiology of disease
ISSN: 1095-953X
Titre abrégé: Neurobiol Dis
Pays: United States
ID NLM: 9500169

Informations de publication

Date de publication:
07 2020
Historique:
received: 20 12 2019
revised: 01 03 2020
accepted: 24 03 2020
pubmed: 31 3 2020
medline: 9 7 2021
entrez: 31 3 2020
Statut: ppublish

Résumé

Alzheimer's disease (AD) is the most common cause of dementia. At the pre-symptomatic phase of the disease, the processing of the amyloid precursor protein (APP) produces toxic peptides, called amyloid-β 1-42 (Aβ 1-42). The downstream effects of Aβ 1-42 production are not completely uncovered. Here, we report the involvement of transglutaminase 1 (TG1) in in vitro AD models of neuronal toxicity. TG1 was increased at late stages of the disease in the hippocampus of a mouse model of AD and in primary cortical neurons undergoing stress. Silencing of TGM1 gene was sufficient to prevent Aβ-mediated neuronal death. Conversely, its overexpression enhanced cell death. TGM1 upregulation was mediated at the transcriptional level by an activator protein 1 (AP1) binding site that when mutated halted TGM1 promoter activation. These results indicate that TG1 acts downstream of Aβ-toxicity, and that its stress-dependent increase makes it suitable for pharmacological intervention.

Identifiants

pubmed: 32222473
pii: S0969-9961(20)30124-8
doi: 10.1016/j.nbd.2020.104849
pii:
doi:

Substances chimiques

Amyloid beta-Peptides 0
Amyloid beta-Protein Precursor 0
Peptide Fragments 0
amyloid beta-protein (1-42) 0
Transglutaminases EC 2.3.2.13
transglutaminase 1 EC 2.3.2.13

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104849

Informations de copyright

Copyright © 2020. Published by Elsevier Inc.

Auteurs

Debasmita Tripathy (D)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy.

Alice Migazzi (A)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy.

Federica Costa (F)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy.

Alessandro Roncador (A)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy.

Pamela Gatto (P)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy.

Federica Fusco (F)

Department of Neuroscience, Laboratory of Genetics of Neurodegenerative Disorders, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milano, Italy.

Lucia Boeri (L)

Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Milan, Italy.

Diego Albani (D)

Department of Neuroscience, Laboratory of Genetics of Neurodegenerative Disorders, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milano, Italy.

J Leon Juárez-Hernández (JL)

Institute of Biophysics, Trento Unit, National Research Council (IBF-CNR), Bruno Kessler Foundation (FBK), LabSSAH, Via alla Cascata 56/C, 38123 Trento, Italy.

Carlo Musio (C)

Institute of Biophysics, Trento Unit, National Research Council (IBF-CNR), Bruno Kessler Foundation (FBK), LabSSAH, Via alla Cascata 56/C, 38123 Trento, Italy.

Laura Colombo (L)

Department of Molecular Biochemistry and Pharmacology, Laboratory of Biochemistry and Protein Chemistry, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milano, Italy.

Mario Salmona (M)

Department of Molecular Biochemistry and Pharmacology, Laboratory of Biochemistry and Protein Chemistry, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milano, Italy.

M M Micha Wilhelmus (MMM)

VU University Medical Center, Neuroscience Campus Amsterdam, Department of Anatomy and Neurosciences, Amsterdam, the Netherlands.

Benjamin Drukarch (B)

VU University Medical Center, Neuroscience Campus Amsterdam, Department of Anatomy and Neurosciences, Amsterdam, the Netherlands.

Maria Pennuto (M)

Dulbecco Telethon Institute Lab of Neurodegenerative Diseases, Centre for Integrative Biology (CIBIO), University of Trento, Italy; Department of Biomedical sciences, via Ugo Bassi 58/B, University of Padova, 35131 Padova, Italy; Padova Neuroscience Center, 35100 Padova, Italy.

Manuela Basso (M)

Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, TN, Italy. Electronic address: manuela.basso@unitn.it.

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Classifications MeSH