Effect of breed body-size on leptin amniotic fluid concentrations at term pregnancy in dogs.


Journal

Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 19 08 2019
revised: 13 02 2020
accepted: 12 03 2020
pubmed: 1 4 2020
medline: 22 1 2021
entrez: 1 4 2020
Statut: ppublish

Résumé

Because of the need to improve the knowledge about canine perinatology, and given the major role of fetal fluids in sustaining the course of pregnancy and fetal development, an in-depth analysis to better understand the role of some hormones in these compartments is essential. Among all, leptin is recognized to play a key role not only on the energetic homeostasis, but also at multiple levels, influencing the control of reproduction, food assumption and metabolism. Even if in humans and other species it is reported the presence of leptin receptors during fetal development, very little is known about the canine species, in which the role of leptin still needs to be fully understood. The present study aimed to assess the amniotic fluid leptin (AFL) concentrations at term pregnancy in healthy dogs, and to evaluate the possible influence played by breed body-size (after assessment of correlation with maternal bodyweight and placental weight), or other maternal (age, parity, and the so-called "litter effect") and neonatal (gender, birth weight, litter size) parameters on AFL concentrations, analyzed by ELISA test. The study was performed on 90 healthy, viable and normal weighted puppies, 39 small-sized (adult body weight < 10 kg) and 51 large-sized (adult body weight > 25 kg), born by 29 purebred, healthy bitches, submitted to elective Caesarean section because of breed-related or individual high risk for dystocia. The results showed that the mean AFL concentration in the small-sized puppies was significantly (p < 0.05) higher in comparison to large-sized puppies (867.48 vs 698.42 pg/ml), while all the other studied parameters did not show to influence AFL concentrations. In conclusions, the present study showed significant higher at term AFL concentrations in small-sized as compared to large-sized breeds, suggesting an influence of breed body-size on fetal metabolism, as previously reported for NEFA and IGF-I.

Identifiants

pubmed: 32224377
pii: S0093-691X(20)30187-4
doi: 10.1016/j.theriogenology.2020.03.021
pii:
doi:

Substances chimiques

Leptin 0

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-5

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest None.

Auteurs

M C Veronesi (MC)

Department of Veterinary Medicine, Università degli Studi di Milano, Via dell'Università 6, Lodi, Italy. Electronic address: maria.veronesi@unimi.it.

J Fusi (J)

Department of Veterinary Medicine, Università degli Studi di Milano, Via dell'Università 6, Lodi, Italy.

A Comin (A)

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio, 2/a, Udine, Italy.

P G Ferrario (PG)

Max Rubner-Institut, Department of Physiology and Biochemistry of Nutrition, Haid-und-Neu-Str. 9, Karlsruhe, Germany.

B Bolis (B)

Department of Veterinary Medicine, Università degli Studi di Milano, Via dell'Università 6, Lodi, Italy.

A Prandi (A)

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio, 2/a, Udine, Italy.

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Classifications MeSH