Salivary Histatin 1 and 2 Are Targeted to Mitochondria and Endoplasmic Reticulum in Human Cells.
Amino Acid Sequence
Cell Line
Endoplasmic Reticulum
/ drug effects
Golgi Apparatus
/ drug effects
Histatins
/ chemistry
Humans
Intercellular Signaling Peptides and Proteins
/ pharmacology
Lysosomes
/ drug effects
Mitochondria
/ drug effects
Models, Biological
Mutant Proteins
/ metabolism
Protein Transport
/ drug effects
Recombinant Proteins
/ pharmacology
Saliva
/ metabolism
Subcellular Fractions
/ metabolism
endoplasmic reticulum
histatin
mitochondria
oral saliva
uptake
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
26 03 2020
26 03 2020
Historique:
received:
10
01
2020
revised:
20
03
2020
accepted:
25
03
2020
entrez:
1
4
2020
pubmed:
1
4
2020
medline:
26
2
2021
Statut:
epublish
Résumé
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their uptake and association with subcellular targets may play a determinant role in their functions. In this study, we studied the uptake dynamics and subcellular targets of Hst1, Hst2 and Hst5 in epithelial cells (HO1N1 human buccal carcinoma epithelial cell line). Confocal laser scanning microscopy (CLSM) revealed that fluorescently labeled Hst1 (F-Hst1) was taken up into the intracellular space of epithelial cells. Then, 60 min post-incubation, the total fluorescence of cell-associated F-Hst1, as measured using flow cytometry, was significantly higher compared to those of F-Hst2 and F-Hst5. In contrast, virtually no association occurred using the negative control-scrambled F-Hst1 (F-Hst
Identifiants
pubmed: 32225006
pii: cells9040795
doi: 10.3390/cells9040795
pmc: PMC7226139
pii:
doi:
Substances chimiques
Histatins
0
Intercellular Signaling Peptides and Proteins
0
Mutant Proteins
0
Recombinant Proteins
0
HTN1 protein, human
101056-53-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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