A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states.
Chemometrics
Metal ions
Metallomics
Voltammetry
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
11
01
2020
revised:
09
03
2020
accepted:
09
03
2020
pubmed:
2
4
2020
medline:
9
1
2021
entrez:
2
4
2020
Statut:
ppublish
Résumé
We focused on the application of mass spectrometry and electrochemical methods combined with a chemometric analysis for the characterization of partially metallothionein-3 species. The results showed decreased Cat1 and Cat2 signals for the Zn(II)-loaded MT3 species with respect to the metal-free protein, which might be explained by the arrangement of tetrahedral metal-thiolate coordination environments and the formation of metal clusters. Moreover, there was a decrease in the Cat1 and Cat2 signals, and a plateau was reached with 4-5 Zn(II) ions that corresponded to the formation of the C-terminal α-domain. Regarding the Zn
Identifiants
pubmed: 32229323
pii: S1567-5394(20)30022-0
doi: 10.1016/j.bioelechem.2020.107501
pii:
doi:
Substances chimiques
Apoproteins
0
Metallothionein 3
0
Nerve Tissue Proteins
0
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107501Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.