Lessons Learned from Somatic Cell Nuclear Transfer.
ESC
SCNT
cloning
enucleation
epigenetic reprogramming
nuclear reprogramming
nuclear transfer
oocyte
somatic cell
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Mar 2020
27 Mar 2020
Historique:
received:
18
02
2020
revised:
16
03
2020
accepted:
19
03
2020
entrez:
2
4
2020
pubmed:
2
4
2020
medline:
6
1
2021
Statut:
epublish
Résumé
Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. However, the consensus is that the low efficiency in creating normal viable offspring in animals by SCNT (1-5%) and the high number of abnormalities seen in these cloned animals is due to epigenetic reprogramming failure. In this review we provide an overview of the current literature on SCNT, focusing on protocol development, which includes early SCNT protocol deficiencies and optimizations along with donor cell type and cell cycle synchrony; epigenetic reprogramming in SCNT; current protocol optimizations such as nuclear reprogramming strategies that can be applied to improve epigenetic reprogramming by SCNT; applications of SCNT; the ethical and legal implications of SCNT in humans; and specific lessons learned for establishing an optimized SCNT protocol using a mouse model.
Identifiants
pubmed: 32230814
pii: ijms21072314
doi: 10.3390/ijms21072314
pmc: PMC7177533
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : South African Medical Research Council
ID : Flagship Grant and Extramural Unit for Stem Cell Research and Therapy
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