Image-Based Dynamic Phenotyping Reveals Genetic Determinants of Filamentation-Mediated β-Lactam Tolerance.
antibiotic tolerance
bacteriolysis
filamentation
high-throughput microscopy
β-lactam
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
31
10
2019
accepted:
19
02
2020
entrez:
2
4
2020
pubmed:
2
4
2020
medline:
2
4
2020
Statut:
epublish
Résumé
Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance. β-lactam antibiotics inhibit cell wall growth in bacteria and many of them cause filamentation followed by cell lysis. Hence delayed cell lysis can lead to β-lactam tolerance. Systematic discovery of genetic factors that affect β-lactam killing kinetics has not been performed before due to challenges in high-throughput, dynamic analysis of viability of filamented cells during bactericidal action. We implemented a high-throughput time-resolved microscopy approach in a gene deletion library of
Identifiants
pubmed: 32231648
doi: 10.3389/fmicb.2020.00374
pmc: PMC7082316
doi:
Types de publication
Journal Article
Langues
eng
Pagination
374Informations de copyright
Copyright © 2020 Zahir, Wilmaerts, Franke, Weytjens, Camacho, Marchal, Hofkens, Fauvart and Michiels.
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