Multigenerational and transgenerational effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on ovarian reserve and follicular development through AMH/AMHR2 pathway in adult female rats.
Animals
Anti-Mullerian Hormone
/ genetics
Female
Male
Ovarian Follicle
/ drug effects
Ovarian Reserve
/ drug effects
Polychlorinated Dibenzodioxins
/ toxicity
Pregnancy
RNA, Messenger
/ genetics
Rats
Rats, Sprague-Dawley
Receptors, Peptide
/ genetics
Receptors, Transforming Growth Factor beta
/ genetics
2,3,7,8-Tetrachlorodibenzo-p-dioxin
Anti-Müllerian hormone
Multigenerational
Ovarian impairment
Transgenerational
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
11
09
2019
revised:
20
03
2020
accepted:
25
03
2020
pubmed:
3
4
2020
medline:
20
2
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
2,3,7,8-Tetrachlorobenzo-p-dioxin (TCDD), one of the key endocrine disruptors, has been shown to cause reproductive and developmental disorders. Our previous studies have primarily focused on TCDD induced impairment of ovarian follicular development in female F1 rats. It is unknown whether TCDD exposure will interfere with follicular development by altering mRNA expression of anti-Müllerian hormone (AMH) and AMH receptor type II (AMHR2) in the ovary. In the present study, pregnant Sprague Dawley rats were treated with TCDD (100 or 500 ng/kg body weight) dissolved in a corn oil vehicle by gavage from gavage from gestational days (GD) 8-14, while the control group received solely corn oil. The F1 rats were mated with unexposed males for the F2 generation, while another portion of the female offspring (F2) were mated for the F3 generation. Serum AMH levels and ovarian AMH/AMHR2 mRNA expression in the adult female offspring (F1, F2 and F3 generations) were measured. Follicle count and granulosa cell apoptosis were evaluated in the F2 and F3 generations. The results showed that in the F2 generation, TCDD exposure affected the number of primordial follicles, secondary follicles, and corpora lutea. It also increased serum AMH concentration and the apoptosis rate of granulosa cells. These results might be associated with the upregulation of AMH/AMHR2 mRNA expression in the ovary. In conclusion, TCDD exposure reduced the ovarian reserve in rats and inhibited follicular development in adult female offspring, an effect that persisted for multiple generations. The altered AMH and AMHR2 mRNA expression may contribute to the observed adverse effects.
Identifiants
pubmed: 32234510
pii: S0278-6915(20)30197-6
doi: 10.1016/j.fct.2020.111309
pii:
doi:
Substances chimiques
Polychlorinated Dibenzodioxins
0
RNA, Messenger
0
Receptors, Peptide
0
Receptors, Transforming Growth Factor beta
0
anti-Mullerian hormone receptor
0
Anti-Mullerian Hormone
80497-65-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111309Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.