Antigenic properties of VP15 from white spot syndrome virus in kuruma shrimp Marsupenaeus japonicus.

Animals Bombyx / growth & development Escherichia coli / genetics Host-Pathogen Interactions Larva / growth & development Nucleocapsid Proteins / immunology Penaeidae / immunology Protective Agents White spot syndrome virus 1 / immunology
Shrimp Silkworm Trained immunity VP15 White spot syndrome virus

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 17 01 2020
revised: 25 03 2020
accepted: 27 03 2020
pubmed: 3 4 2020
medline: 13 1 2021
entrez: 3 4 2020
Statut: ppublish

Résumé

White spot syndrome virus (WSSV) is known as one of the most lethal pathogenic viruses in shrimp causing massive damage to shrimp aquaculture industries. To date, no effective treatment or prevention has been found. In this study, five recombinant viral proteins VP15, VP19, VP24, VP26, and VP28 were expressed and purified in E. coli, which were employed as candidates against WSSV in Kuruma shrimp Marsupenaeus japonicus. In vivo antiviral assay in this study newly revealed that VP15 of major nucleocapsid protein, being known as a DNA-binding protein provided the substantial protection against the viral infection when pre-injected into shrimps. Furthermore, we also verified the immunogenic effects of purified VP15 and VP19 proteins produced in a silkworm-bacmid expression system. Taken together, our study identified VP15 as an effective candidate against WSSV infection in the Kuruma shrimp. It is interesting to uncover why and how VP15 is involved in the immune memory in shrimp in the future study.

Identifiants

DOI: 10.1016/j.fsi.2020.03.061 PMID: 32234560
pubmed: 32234560
pii: S1050-4648(20)30229-1
doi: 10.1016/j.fsi.2020.03.061
pii:
doi:

Substances chimiques

Nucleocapsid Proteins 0
Protective Agents 0
VP15 protein, white spot syndrome virus 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

152-158

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no conflict of interest.

Auteurs

Jirayu Boonyakida (J)

Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: jirayu.boonyakida.17@shizuoka.ac.jp.

Jian Xu (J)

Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: xu.jian@shizuoka.ac.jp.

Jun Satoh (J)

National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Saiki, Oita, Japan. Electronic address: junsatoh@affrc.go.jp.

Takafumi Nakanishi (T)

Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: nakanishi19951203@gmail.com.

Toru Mekata (T)

National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Saiki, Oita, Japan. Electronic address: mekata@affrc.go.jp.

Tatsuya Kato (T)

Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: kato.tatsuya@shizuoka.ac.jp.

Enoch Y Park (EY)

Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Lepidoptera › Papillons de nuit › Bombyx › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Crustacea › Decapoda (crustacea) › Penaeidae › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antigenic properties of VP15 from white spot...
questionsmedicales.fr › Virus › Virus à ADN › Nimaviridae › Virus de type 1 du syndrome des taches blanches › Antigenic properties of VP15 from white spot...