Long non-coding RNA CRNDE deteriorates intrauterine infection-induced neonatal brain injury.
Animals
Animals, Newborn
Astrocytes
/ pathology
Brain
/ pathology
Brain Injuries
/ etiology
Cell Death
Cytokines
/ biosynthesis
Female
Gene Knockdown Techniques
Humans
Lipopolysaccharides
Male
Memory
Microglia
/ pathology
Pregnancy
RNA, Long Noncoding
/ genetics
Rats
Spatial Learning
Up-Regulation
/ genetics
Uterus
/ microbiology
Brain injury
Intrauterine infection
Lipopolysaccharide
Neonatal rats
lncRNA CRNDE
Journal
Molecular and cellular probes
ISSN: 1096-1194
Titre abrégé: Mol Cell Probes
Pays: England
ID NLM: 8709751
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
27
12
2019
revised:
09
03
2020
accepted:
24
03
2020
pubmed:
3
4
2020
medline:
3
6
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats. Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia. LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS. CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.
Sections du résumé
BACKGROUND
This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats.
METHODS
Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia.
RESULTS
LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS.
CONCLUSION
CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.
Identifiants
pubmed: 32234564
pii: S0890-8508(19)30506-7
doi: 10.1016/j.mcp.2020.101565
pii:
doi:
Substances chimiques
Cytokines
0
Lipopolysaccharides
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
101565Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest All the authors declare no conflict of interest.