Knockdown of CARD14 Inhibits Cell Proliferation and Migration in Breast Cancer Cells.
Apoptosis
/ genetics
Breast Neoplasms
/ genetics
CARD Signaling Adaptor Proteins
/ genetics
Cell Cycle
/ genetics
Cell Cycle Checkpoints
/ genetics
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Female
Gene Expression Regulation, Neoplastic
/ genetics
Gene Knockdown Techniques
Guanylate Cyclase
/ genetics
Humans
MCF-7 Cells
Membrane Proteins
/ genetics
RNA, Small Interfering
/ genetics
CARD14
apoptosis
breast cancer
migration
proliferation
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
17
02
2020
revised:
24
02
2020
accepted:
25
02
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
10
4
2020
Statut:
ppublish
Résumé
Caspase recruitment domain family, member 14 (CARD14) is a member of the CARD family of proteins, which play an important role in immune and inflammatory response, and cell survival and proliferation. Here, we identified the role of CARD14 in human breast cancer. Immunohistochemistry was performed to evaluate CARD14 expression in breast cancer. Using CARD14 knockdown cells by small interfering RNA, colony formation and MTT assays, flow cytometry analyses, and migration assays were performed to evaluate the proliferation, cell cycle distribution, apoptosis, and migration ability of MCF7 and SK-BR-3 cells. CARD14 expression was significantly higher in breast cancer samples than in normal breast samples. CARD14 knockdown inhibited cell proliferation and migration, caused cell cycle arrest at the G CARD14 regulates the proliferation and migration of MCF7 and SK-BR-3 cells; it is thus, a novel potential therapeutic target in breast cancer.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Caspase recruitment domain family, member 14 (CARD14) is a member of the CARD family of proteins, which play an important role in immune and inflammatory response, and cell survival and proliferation. Here, we identified the role of CARD14 in human breast cancer.
MATERIALS AND METHODS
METHODS
Immunohistochemistry was performed to evaluate CARD14 expression in breast cancer. Using CARD14 knockdown cells by small interfering RNA, colony formation and MTT assays, flow cytometry analyses, and migration assays were performed to evaluate the proliferation, cell cycle distribution, apoptosis, and migration ability of MCF7 and SK-BR-3 cells.
RESULTS
RESULTS
CARD14 expression was significantly higher in breast cancer samples than in normal breast samples. CARD14 knockdown inhibited cell proliferation and migration, caused cell cycle arrest at the G
CONCLUSION
CONCLUSIONS
CARD14 regulates the proliferation and migration of MCF7 and SK-BR-3 cells; it is thus, a novel potential therapeutic target in breast cancer.
Identifiants
pubmed: 32234884
pii: 40/4/1953
doi: 10.21873/anticanres.14150
doi:
Substances chimiques
CARD Signaling Adaptor Proteins
0
Membrane Proteins
0
RNA, Small Interfering
0
CARD14 protein, human
EC 4.6.1.-
Guanylate Cyclase
EC 4.6.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1953-1962Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.