Oropharyngeal HPV Detection Techniques in HPV-associated Head and Neck Cancer Patients.
HNSCC
HPV
HPV testing
Head and neck cancer
Human papillomavirus
OPSCC
oral rinse
oropharyngeal brushing
oropharyngeal carcinoma
oropharyngeal infection
sexually transmitted disease
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
21
02
2020
revised:
09
03
2020
accepted:
11
03
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
11
4
2020
Statut:
ppublish
Résumé
The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention. Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue. Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods. Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention.
PATIENTS AND METHODS
METHODS
Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue.
RESULTS
RESULTS
Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods.
CONCLUSION
CONCLUSIONS
Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended.
Identifiants
pubmed: 32234904
pii: 40/4/2117
doi: 10.21873/anticanres.14170
doi:
Substances chimiques
DNA, Viral
0
Mouthwashes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2117-2123Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.