Elevated Lactate Levels in Acute Pulmonary Embolism Are Associated with Prothrombotic Fibrin Clot Properties: Contribution of NETs Formation.

NETosis acute thrombosis fibrinolysis lactate pulmonary embolism

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
30 Mar 2020
Historique:
received: 28 02 2020
revised: 24 03 2020
accepted: 27 03 2020
entrez: 3 4 2020
pubmed: 3 4 2020
medline: 3 4 2020
Statut: epublish

Résumé

Elevated plasma lactate levels correlate with high mortality rate in acute pulmonary embolism (PE) patients. We hypothesized that elevated lactate levels correlate with prothrombotic fibrin clot properties and enhanced neutrophil extracellular trap (NET) formation in acute PE. As many as 126 normotensive acute PE patients (aged 58 ± 14 years) were enrolled. Plasma fibrin clot permeability (K Lactate levels ≥2 mM were found in 70 (55.6%) patients in whom we observed 29% higher neutrophil count and 45% elevated plasma citH3 levels. Elevated lactate levels were associated with more prothrombotic fibrin properties as reflected by 11% reduced K Our findings suggest a new mechanism contributing to a negative impact of elevated lactate levels on prognosis in acute PE patients, in particular hypofibrinolysis, associated with enhanced NET formation.

Sections du résumé

BACKGROUND BACKGROUND
Elevated plasma lactate levels correlate with high mortality rate in acute pulmonary embolism (PE) patients. We hypothesized that elevated lactate levels correlate with prothrombotic fibrin clot properties and enhanced neutrophil extracellular trap (NET) formation in acute PE.
METHODS METHODS
As many as 126 normotensive acute PE patients (aged 58 ± 14 years) were enrolled. Plasma fibrin clot permeability (K
RESULTS RESULTS
Lactate levels ≥2 mM were found in 70 (55.6%) patients in whom we observed 29% higher neutrophil count and 45% elevated plasma citH3 levels. Elevated lactate levels were associated with more prothrombotic fibrin properties as reflected by 11% reduced K
CONCLUSIONS CONCLUSIONS
Our findings suggest a new mechanism contributing to a negative impact of elevated lactate levels on prognosis in acute PE patients, in particular hypofibrinolysis, associated with enhanced NET formation.

Identifiants

pubmed: 32235490
pii: jcm9040953
doi: 10.3390/jcm9040953
pmc: PMC7231195
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Narodowe Centrum Nauki
ID : UMO-2015/B/NZ5/02215

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

Références

Arterioscler Thromb Vasc Biol. 2011 Dec;31(12):e88-99
pubmed: 21836064
J Clin Invest. 2002 May;109(10):1311-9
pubmed: 12021246
Free Radic Biol Med. 2016 Apr;93:190-203
pubmed: 26774674
Med Oncol. 2019 Mar 19;36(4):37
pubmed: 30891644
Sci Rep. 2018 Nov 23;8(1):17301
pubmed: 30470809
PLoS One. 2012;7(10):e48111
pubmed: 23110185
Blood. 2018 Feb 15;131(7):797-807
pubmed: 29242187
Circ Res. 2016 Apr 29;118(9):1340-7
pubmed: 27126645
Front Immunol. 2016 Dec 05;7:565
pubmed: 27994595
Ann Emerg Med. 2013 Mar;61(3):330-8
pubmed: 23306454
Subcell Biochem. 2017;82:405-456
pubmed: 28101869
J Thromb Haemost. 2018 Mar;16(3):508-518
pubmed: 29325226
J Thromb Haemost. 2014 Jun;12(6):860-70
pubmed: 24674135
J Exp Med. 2012 Apr 9;209(4):819-35
pubmed: 22451716
Blood. 2010 Jul 8;116(1):113-21
pubmed: 20385790
J Proteomics. 2019 Sep 30;208:103487
pubmed: 31425886
Free Radic Biol Med. 2009 Oct 15;47(8):1140-6
pubmed: 19631267
Physiology (Bethesda). 2018 Jan 1;33(1):10-12
pubmed: 29212886
Free Radic Biol Med. 2012 Jul 15;53(2):230-6
pubmed: 22580301
Blood Coagul Fibrinolysis. 2009 Jan;20(1):12-6
pubmed: 20527721
Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):1977-84
pubmed: 25012129
Eur Heart J. 2015 Jun 7;36(22):1405-14
pubmed: 25660055
Mayo Clin Proc. 2013 Oct;88(10):1127-40
pubmed: 24079682
Biochim Biophys Acta Mol Basis Dis. 2019 Dec 1;1865(12):165542
pubmed: 31473341
Eur Respir J. 2019 Oct 9;54(3):
pubmed: 31473594
N Engl J Med. 2014 Dec 11;371(24):2309-19
pubmed: 25494270
J Thromb Haemost. 2011 Sep;9(9):1795-803
pubmed: 21711444
Kardiol Pol. 2019;77(3):355-362
pubmed: 30761511
Thromb Haemost. 2007 Oct;98(4):756-64
pubmed: 17938798
Arterioscler Thromb Vasc Biol. 2019 Sep;39(9):1724-1738
pubmed: 31315434
Tumour Biol. 2016 Nov;37(11):14355-14361
pubmed: 27614687
Int Immunol. 2020 May 8;32(5):359-368
pubmed: 31879779
J Thromb Haemost. 2018 May;16(5):1007-1012
pubmed: 29658191
Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15880-5
pubmed: 20798043
Thromb Res. 2017 Aug;156:26-28
pubmed: 28582637
Cardiovasc Diabetol. 2019 Apr 16;18(1):49
pubmed: 30992036
Thorax. 2015 Apr;70(4):333-8
pubmed: 25661114
J Biol Chem. 2013 Mar 8;288(10):6946-56
pubmed: 23293023
Nitric Oxide. 2010 Apr 1;22(3):226-34
pubmed: 20060922
Front Immunol. 2017 Aug 15;8:975
pubmed: 28861083

Auteurs

Michał Ząbczyk (M)

Institute of Cardiology, Jagiellonian University Medical College, 31-202 Krakow, Poland.
John Paul II Hospital, 31-202 Krakow, Poland.

Joanna Natorska (J)

Institute of Cardiology, Jagiellonian University Medical College, 31-202 Krakow, Poland.
John Paul II Hospital, 31-202 Krakow, Poland.

Agnieszka Janion-Sadowska (A)

The Faculty of Medicine and Health Sciences, The Jan Kochanowski University, 25-317 Kielce, Poland.

Krzysztof P Malinowski (KP)

Institute of Public Health, Faculty of Health Sciences, Jagiellonian University Medical College, 31-126 Krakow, Poland.

Marianna Janion (M)

The Faculty of Medicine and Health Sciences, The Jan Kochanowski University, 25-317 Kielce, Poland.

Anetta Undas (A)

Institute of Cardiology, Jagiellonian University Medical College, 31-202 Krakow, Poland.
John Paul II Hospital, 31-202 Krakow, Poland.
The Faculty of Medicine and Health Sciences, The Jan Kochanowski University, 25-317 Kielce, Poland.

Classifications MeSH