Microtubule-associated protein 2 mediates induction of long-term potentiation in hippocampal neurons.
Animals
CA1 Region, Hippocampal
/ cytology
Cells, Cultured
Dendritic Spines
/ metabolism
In Vitro Techniques
Long-Term Potentiation
/ physiology
MAP Kinase Signaling System
Male
Mice, Inbred C57BL
Microscopy, Immunoelectron
Microtubule-Associated Proteins
/ antagonists & inhibitors
Neuronal Plasticity
/ physiology
Protein Transport
Pyramidal Cells
/ metabolism
RNA Interference
RNA, Small Interfering
/ genetics
Rats
Receptors, AMPA
/ metabolism
MAP2
dendritic spine
electron microscopy
synaptic plasticity
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
19
08
2019
revised:
16
03
2020
accepted:
17
03
2020
pubmed:
3
4
2020
medline:
28
1
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
Microtubule-associated protein (MAP) 2 has been perceived as a static cytoskeletal protein enriched in neuronal dendritic shafts. Emerging evidence indicates dynamic functions for various MAPs in activity-dependent synaptic plasticity. However, it is unclear how MAP2 is associated with synaptic plasticity mechanisms. Here, we demonstrate that specific silencing of high-molecular-weight MAP2 in vivo abolished induction of long-term potentiation (LTP) in the Schaffer collateral pathway of CA1 pyramidal neurons and in vitro blocked LTP-induced surface delivery of AMPA receptors and spine enlargement. In mature hippocampal neurons, we observed rapid translocation of a subpopulation of MAP2, present in dendritic shafts, to spines following LTP stimulation. Time-lapse confocal imaging showed that spine translocation of MAP2 was coupled with LTP-induced spine enlargement. Consistently, immunogold electron microscopy revealed that LTP stimulation of the Schaffer collateral pathway promoted MAP2 labeling in spine heads of CA1 neurons. This translocation depended on NMDA receptor activation and Ras-MAPK signaling. Furthermore, LTP stimulation led to an increase in surface-expressed AMPA receptors specifically in the neurons with MAP2 spine translocation. Altogether, this study indicates a novel role for MAP2 in LTP mechanisms and suggests that MAP2 participates in activity-dependent synaptic plasticity in mature hippocampal networks.
Identifiants
pubmed: 32237183
doi: 10.1096/fj.201902122RR
doi:
Substances chimiques
MAP2 protein, rat
0
Microtubule-Associated Proteins
0
RNA, Small Interfering
0
Receptors, AMPA
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6965-6983Informations de copyright
© 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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