Oxygen Surrogate Systems for Supporting Human Drug-Metabolizing Cytochrome P450 Enzymes.
Journal
Drug metabolism and disposition: the biological fate of chemicals
ISSN: 1521-009X
Titre abrégé: Drug Metab Dispos
Pays: United States
ID NLM: 9421550
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
20
01
2020
accepted:
12
03
2020
pubmed:
3
4
2020
medline:
20
7
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
Oxygen surrogates (OSs) have been used to support cytochrome P450 (P450) enzymes for diverse purposes in drug metabolism research, including reaction phenotyping, mechanistic and inhibition studies, studies of redox partner interactions, and to avoid the need for NADPH or a redox partner. They also have been used in engineering P450s for more cost-effective, NADPH-independent biocatalysis. However, despite their broad application, little is known of the preference of individual P450s for different OSs or the substrate dependence of OS-supported activity. Furthermore, the biocatalytic potential of OSs other than cumene hydroperoxide (CuOOH) and hydrogen peroxide (H
Identifiants
pubmed: 32238418
pii: dmd.120.090555
doi: 10.1124/dmd.120.090555
doi:
Substances chimiques
Recombinant Proteins
0
Cytochrome P-450 Enzyme System
9035-51-2
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
432-437Informations de copyright
Copyright © 2020 by The American Society for Pharmacology and Experimental Therapeutics.