Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
05
08
2019
accepted:
12
03
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
7
7
2020
Statut:
epublish
Résumé
Pericellular and extracellular proteoglycans play an important role in modulating morphogen gradients and signal transductions. Chondroitin sulfate proteoglycan 4 (Cspg4) is a membrane spanning proteoglycan expressed in immature progenitor cells and cancer cells. Cspg4 participates in cellular events such as proliferation, migration and signal transduction, and these events are generally important for embryo development. In this study, we characterized Cspg4 for its roles in zebrafish embryonic development. Our results demonstrated that cspg4 was maternally expressed from 0 to 3 hours post fertilization (hpf) and expressed in the anterior and posterior embryo end after 9 hpf. Knocking-down cspg4 resulted in a shorter anterior-posterior axis than control embryo, which could be rescued by co-injecting wnt11 mRNA suggesting that Cspg4 regulates body axis organization through modulating the Wnt/planar cell polarity signaling pathway. In addition, overexpressing cspg4 caused cyclopia. The Cspg4 transmembrane domain mutant embryo phenocopied the global over-expression of cspg4 mRNA and led to cyclopia with a very low penetrance. Our results demonstrated that the quantitatively and spatially accurate distribution of Cspg4 is critical for body axis and midline development during gastrulation.
Identifiants
pubmed: 32240230
doi: 10.1371/journal.pone.0230943
pii: PONE-D-19-22022
pmc: PMC7117731
doi:
Substances chimiques
Antigens
0
Proteoglycans
0
RNA, Messenger
0
chondroitin sulfate proteoglycan 4
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0230943Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cancer Res. 2009 Oct 1;69(19):7538-47
pubmed: 19738072
PLoS Genet. 2007 May 25;3(5):e78
pubmed: 17530925
Cytotherapy. 2009;11(5):527-33
pubmed: 19462316
J Biol Chem. 2009 Apr 24;284(17):11728-37
pubmed: 19211552
J Cell Biol. 2004 Jun 21;165(6):881-91
pubmed: 15210734
Nat Cell Biol. 1999 Dec;1(8):507-13
pubmed: 10587647
Development. 2004 Feb;131(3):525-37
pubmed: 14729574
PLoS One. 2014 May 29;9(5):e98186
pubmed: 24873830
Development. 2000 Mar;127(5):921-32
pubmed: 10662632
J Neurocytol. 2002 Jul-Aug;31(6-7):423-35
pubmed: 14501214
Development. 2003 Nov;130(22):5375-84
pubmed: 13129848
Cold Spring Harb Perspect Biol. 2009 Sep;1(3):a002493
pubmed: 20066107
Dev Cell. 2001 Aug;1(2):251-64
pubmed: 11702784
J Biol Chem. 2008 Mar 28;283(13):8310-7
pubmed: 18218632
Nucleic Acids Res. 2017 Jan 4;45(D1):D190-D199
pubmed: 27899635
Transl Lung Cancer Res. 2013 Apr;2(2):87-91
pubmed: 25806219
Nat Commun. 2013;4:2803
pubmed: 24335906
Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57
pubmed: 22004131
Nat Biotechnol. 2018 Nov 27;:
pubmed: 30480667
PLoS One. 2016 Dec 9;11(12):e0167644
pubmed: 27936201
Genes Dev. 2005 Jan 1;19(1):164-75
pubmed: 15630025
Curr Biol. 2003 Aug 5;13(15):1279-89
pubmed: 12906787
Biochem J. 2007 May 1;403(3):553-63
pubmed: 17217338
Elife. 2017 Nov 16;6:
pubmed: 29144233
J Biol Chem. 2010 Apr 16;285(16):12190-6
pubmed: 20164174
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9710-5
pubmed: 8790396
Curr Opin Cell Biol. 2018 Apr;51:50-57
pubmed: 29153705
J Neurosci. 1987 Sep;7(9):2737-44
pubmed: 3305800
Sci Rep. 2014 Jan 15;4:3701
pubmed: 24424429
J Natl Cancer Inst. 2010 Oct 6;102(19):1496-512
pubmed: 20852124
Development. 1996 Apr;122(4):1313-22
pubmed: 8620858
Dev Dyn. 2001 Oct;222(2):218-27
pubmed: 11668599
J Biol Chem. 1999 Jun 11;274(24):16831-7
pubmed: 10358027
Adv Cancer Res. 2010;109:73-121
pubmed: 21070915
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W401-7
pubmed: 24861617
J Cell Biol. 1991 Jul;114(2):359-71
pubmed: 1906475
Bioinformatics. 2009 May 1;25(9):1189-91
pubmed: 19151095
Development. 2000 Sep;127(18):3899-911
pubmed: 10952888
Int Rev Cytol. 2007;261:159-92
pubmed: 17560282
Exp Cell Res. 1997 Aug 25;235(1):254-64
pubmed: 9281375
Nature. 2000 May 4;405(6782):76-81
pubmed: 10811221
Mech Dev. 2015 Nov;138 Pt 3:279-90
pubmed: 26459057
Cancer Res. 2010 Feb 15;70(4):1645-55
pubmed: 20145126
Development. 1991 Apr;111(4):933-44
pubmed: 1879362
J Cell Sci. 1999 Mar;112 ( Pt 6):905-15
pubmed: 10036240
Dev Cell. 2002 Jun;2(6):695-706
pubmed: 12062082
Amino Acids. 2013 Feb;44(2):615-29
pubmed: 22907836
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
J Biol Chem. 1996 Oct 18;271(42):26110-6
pubmed: 8824254