Survival of Group A Streptococcus (GAS) is Enhanced Under Desiccated Culture Conditions.
Adolescent
Antigens, Bacterial
/ genetics
Bacterial Outer Membrane Proteins
/ genetics
Carrier Proteins
/ genetics
Child
Child, Preschool
Desiccation
Genotype
Humans
Infant
Microbial Viability
Pharyngitis
/ microbiology
Reinfection
/ microbiology
Streptococcal Infections
/ microbiology
Streptococcus pyogenes
/ genetics
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
31
01
2020
accepted:
24
03
2020
pubmed:
3
4
2020
medline:
26
3
2021
entrez:
3
4
2020
Statut:
ppublish
Résumé
Streptococcus pyogenes or Group A Streptococcus (GAS) infections are the leading cause of bacterial tonsillopharyngitis. The bacterium can survive and persist within the human host for a long time as it is observed in up to 40% of the population who are considered as carriers. Recurrent tonsillopharyngitis is a particular problem in children which is caused either by relapses due to failed bacterial clearance or by reinfection. A prolonged survival in tonsillar crypts or on inanimate surfaces might be sources for reinfection. We therefore examined 64 clinical GAS isolates from children with tonsillopharyngitis for their long-term survival under either liquid or desiccated culture conditions. After 6 weeks, the overall GAS survival rate was 400-fold increased under desiccated culture conditions compared to liquid culture conditions, but varied depending on the emm-type between 20-fold (emm4) and 14000-fold (emm3). The survival rates of isolates from emm75 were significantly lower which is probably due to their production of hydrogen peroxide up to fatal doses. No hydrogen peroxide production could be detected for other emm-types. Furthermore, 11 isolates from patients with recurrent tonsillopharyngitis were compared to isolates of the same emm-type from patients with single episodes of tonsillopharyngitis. A significant elevated pH value and an increased survival rate for isolates from patients with recurrent infections were observed. In conclusion, significant differences in long-term survival of different GAS isolates as well as survival under desiccated culture conditions might contribute to both failed bacterial clearance and reinfection in patients with recurrent tonsillopharyngitis.
Identifiants
pubmed: 32240341
doi: 10.1007/s00284-020-01967-8
pii: 10.1007/s00284-020-01967-8
pmc: PMC8238923
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Carrier Proteins
0
streptococcal M protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1518-1524Commentaires et corrections
Type : ErratumIn
Références
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