Beta-estradiol protects against copper-ascorbate induced oxidative damage in goat liver mitochondria in vitro by binding with ascorbic acid.
Animals
Antioxidants
/ pharmacology
Ascorbic Acid
/ adverse effects
Copper
/ adverse effects
Estradiol
/ pharmacology
Female
Glutathione
/ metabolism
Goats
In Vitro Techniques
Lipid Peroxidation
Membrane Potential, Mitochondrial
Mitochondria, Liver
/ drug effects
Oxidation-Reduction
Oxidative Stress
/ drug effects
Permeability
Protein Binding
Antioxidant
Cu(2+)-ascorbate
Fluctuation in heat change (ΔH)
JC-1 dye
Mitochondria
Oxidative stress
β-Estradiol
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
01 Jun 2020
01 Jun 2020
Historique:
received:
31
12
2019
revised:
23
03
2020
accepted:
27
03
2020
pubmed:
3
4
2020
medline:
6
5
2020
entrez:
3
4
2020
Statut:
ppublish
Résumé
β-Estradiol (β-E), one of the chemical forms of female gonad hormone exhibited antioxidant efficacy in biochemical system, in vitro. The aim of the study was to investigate whether any other mechanism of protection by β-E to hepatic mitochondria in presence of stressor agent i.e.,a combination of Cu Freshly prepared goat liver mitochondria was incubated with stressors and 1 μM β-E and post incubated with the same concentration at 37 °C at pH 7.4. Mitochondrial viability, biomarkers of oxidative stress, activities of Krebs cycle enzymes, mitochondrial membrane potential, Ca Upon incubation of mitochondria with Cu, ascorbic acid and their combination there is a significant decline in activities of four of Krebs cycle enzymes in an uncompetitive manner with a concomitant increase in Ca Our results for the first time demonstrated that β-E protects againstCu
Identifiants
pubmed: 32240678
pii: S0024-3205(20)30344-1
doi: 10.1016/j.lfs.2020.117596
pii:
doi:
Substances chimiques
Antioxidants
0
Estradiol
4TI98Z838E
Copper
789U1901C5
Glutathione
GAN16C9B8O
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117596Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest Authors declare that there are no conflicts of interest.