Preparation and Evaluation of BDNF Composite Conduits for Regeneration of Sciatic Nerve Defect in Rats.

Animals Brain-Derived Neurotrophic Factor Nerve Regeneration Rats Rats, Sprague-Dawley Sciatic Nerve
Biodegradable polymers Brain-derived neurotrophic factor Conduits Mechanical properties Nerve regeneration PEG PLA Peripheral nerve injury

Journal

Journal of pharmaceutical sciences
ISSN: 1520-6017
Titre abrégé: J Pharm Sci
Pays: United States
ID NLM: 2985195R

Informations de publication

Date de publication:
07 2020
Historique:
received: 08 12 2019
revised: 22 02 2020
accepted: 17 03 2020
pubmed: 3 4 2020
medline: 22 6 2021
entrez: 3 4 2020
Statut: ppublish

Résumé

The treatment of peripheral nerve injury is still a challenge. The present study was aimed to fabricate a composite nerve conduit containing slow-released brain-derived neurotrophic factor (BDNF) and evaluate its therapeutic effects on peripheral nerve defect. BDNF and polylactic acid were mixed together and a BDNF-loaded composite conduit was fabricated by solvent evaporation method. The bioactivity of BDNF released from the composite conduit was measured by MTT assay. A rat sciatic nerve defect model was used to compare regeneration potentials of autologous nerve graft, conduit filled with saline, conduit filled with BDNF solution and BDNF composite conduit. Nerve-regeneration was evaluated 3 months after surgery. The results demonstrated BDNF composite conduits prepared in this study remained bioactive for at least 3 months, and can promote the regeneration of sciatic nerve with a 10-mm gap in rats.

Identifiants

DOI: 10.1016/j.xphs.2020.03.027 PMID: 32240698
pubmed: 32240698
pii: S0022-3549(20)30186-6
doi: 10.1016/j.xphs.2020.03.027
pii:
doi:

Substances chimiques

Brain-Derived Neurotrophic Factor 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2189-2195

Informations de copyright

Copyright © 2020 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

Auteurs

Huanhuan Su (H)

Department of Pharmaceutics, Chinese PLA General Hospital, Beijing 100853, China.

Fenghua Xu (F)

Department of Pharmaceutics, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: fhxu11@163.com.

Huayan Sun (H)

Department of Pharmaceutics, Chinese PLA General Hospital, Beijing 100853, China.

Xiaofei Fu (X)

Department of Pharmaceutics, Rocket Force Characteristic Medical Center of Chinese People' Liberation Army, Beijing 100088, China.

Yi Zhao (Y)

School of Pharmacy, MCPHS University, Boston, Massachusetts 02115.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Preparation and Evaluation of BDNF Composite...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Facteurs de croissance nerveuse › Facteur neurotrophique dérivé du cerveau › Preparation and Evaluation of BDNF Composite...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Régénération nerveuse › Preparation and Evaluation of BDNF Composite...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Preparation and Evaluation of BDNF Composite...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Preparation and Evaluation of BDNF Composite...
questionsmedicales.fr › Système nerveux › Système nerveux périphérique › Nerfs périphériques › Nerfs spinaux › Plexus lombosacral › Nerf ischiatique › Preparation and Evaluation of BDNF Composite...