Ovary removal modifies liver message RNA profiles in single Comb White Leghorn chickens.
RNA-seq
liver mRNA profile
ovariectomy
single Comb White Leghorn chicken
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
21
10
2018
revised:
29
11
2019
accepted:
02
12
2019
entrez:
4
4
2020
pubmed:
4
4
2020
medline:
21
10
2020
Statut:
ppublish
Résumé
Ovaries produce sex hormones, and ovariectomized animals are often used as models for ovarian dysfunction. The liver is a vital organ involved in metabolism and immunity. In the present study, we conducted experiments to investigate the effects of ovariectomy on transcription and metabolic processes in the liver in chicken. Eight Single Comb White Leghorn (SCWL) female chickens were ovariectomized at 17 wk of age, and 8 intact SCWL females served as controls. At 100 wk of age, all chickens were euthanized. High-throughput transcriptome sequencing was performed on liver RNA obtained from ovariectomized and intact females. A total of 267 differentially expressed genes (DEG) were identified in our study. After analysis using DAVID functional annotation tool, one significant Kyoto Encyclopedia of Genes and Genomes pathway, the phosphatidylinositol signaling pathway, was clustered. Gene Ontology enrichment analysis yielded 46 significant Gene Ontology terms. Among terms describing biological processes, the glycerolipid metabolic and lipid localization processes were dominant. The anabolic genes, PEPCK and GK5, and the catabolic genes, VTG1; VTG2; PLD5; DGKQ; DGKE; and FABP3, were detected in ovariectomized chickens. Differentially expressed genes such as ENSGALG00000000162, IL-1Β, SVOPL, and CA12 implied that livers in ovariectomized chickens were subjected to strong inflammatory reactions, whereas defenses against endogenous materials were compromised. A comprehensive view of gene expression in the liver of ovariectomized chickens would advance our understanding of lipid metabolism, glycometabolism, and their relationships to pathologies induced by absence of the ovary. The identified DEG indicated that ovariectomy disturbed lipid metabolism in the liver and was accompanied by an increase in hepatic gluconeogenesis and reductions in phosphatidic acid synthesis and lipid carrier capacity.
Identifiants
pubmed: 32241461
pii: S0032-5791(20)30009-2
doi: 10.1016/j.psj.2019.12.036
pmc: PMC7587799
pii:
doi:
Substances chimiques
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1813-1821Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1257-63
pubmed: 7802658
Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3008-13
pubmed: 24516121
Meat Sci. 2008 Apr;78(4):343-58
pubmed: 22062452
Mol Biol Rep. 2011 Mar;38(3):2161-6
pubmed: 20848210
J Clin Endocrinol Metab. 2010 May;95(5):2508-12
pubmed: 20228163
Curr Opin Clin Nutr Metab Care. 2014 Mar;17(2):124-9
pubmed: 24500438
World J Gastroenterol. 2004 Jul 1;10(13):1967-70
pubmed: 15222048
Poult Sci. 2017 Mar 1;96(3):770-777
pubmed: 27738117
Children (Basel). 2015 Jul 31;2(3):358-70
pubmed: 27417369
Biochim Biophys Acta. 2010 Oct;1800(10):1030-44
pubmed: 19909788
Am J Physiol Cell Physiol. 2010 Aug;299(2):C335-44
pubmed: 20427710
Genomics. 2007 Nov;90(5):595-609
pubmed: 17714910
J Nutr Sci Vitaminol (Tokyo). 2005 Apr;51(2):110-7
pubmed: 16022198
Transplantation. 2010 Jun 27;89(12):1425-9
pubmed: 20463637
Am J Physiol Endocrinol Metab. 2010 Oct;299(4):E657-64
pubmed: 20682840
Am J Physiol Cell Physiol. 2001 Oct;281(4):C1131-8
pubmed: 11546648
Nutrition. 2009 Jun;25(6):676-85
pubmed: 19230619
J Clin Endocrinol Metab. 2011 Apr;96(4):885-93
pubmed: 21474685
Toxicol Lett. 2015 Jan 5;232(1):253-62
pubmed: 25448287
Cell Signal. 2011 Dec;23(12):1988-96
pubmed: 21802511
J Biol Chem. 1995 Dec 1;270(48):28495-8
pubmed: 7499357
Semin Immunopathol. 2009 Sep;31(3):333-43
pubmed: 19639317
Nature. 2013 Sep 12;501(7466):257-61
pubmed: 23934110
Nutr Metab (Lond). 2015 May 20;12:17
pubmed: 26052340
Mol Endocrinol. 2010 Jul;24(7):1393-403
pubmed: 20501640
BMC Med Genomics. 2013 Mar 19;6:9
pubmed: 23510163
Indian J Endocrinol Metab. 2015 Jan-Feb;19(1):89-94
pubmed: 25593833
Neurosci Res. 1998 Apr;30(4):303-12
pubmed: 9678634
J Clin Invest. 1969 Apr;48(4):696-703
pubmed: 5774107
Dev Cell. 2002 May;2(5):643-53
pubmed: 12015971
Dig Dis Sci. 2012 Jul;57(7):1773-81
pubmed: 22419057
Am J Physiol Regul Integr Comp Physiol. 2015 Dec 15;309(12):R1569-77
pubmed: 26491104
Autophagy. 2015;11(5):785-95
pubmed: 25946330
J Biol Chem. 1990 Oct 25;265(30):18055-8
pubmed: 2211681
J Mol Biol. 1999 Jan 8;285(1):391-408
pubmed: 9878414
PLoS One. 2013;8(3):e59636
pubmed: 23544081
Am J Physiol Regul Integr Comp Physiol. 2013 Sep;305(5):R459-63
pubmed: 23883676
Curr Hypertens Rep. 2014 Jan;16(1):408
pubmed: 24343167
Przegl Lek. 1998;55(5):266-70
pubmed: 9741194
Comp Biochem Physiol B Biochem Mol Biol. 2006 Oct;145(2):147-58
pubmed: 16905349
Diabetologia. 2001 Jun;44(6):659-73
pubmed: 11440359
Endocr Rev. 2008 Oct;29(6):647-76
pubmed: 18606873
J Neuroimmunol. 2010 Feb 26;219(1-2):90-9
pubmed: 20096467
Appl Physiol Nutr Metab. 2009 Dec;34(6):1079-86
pubmed: 20029517
BMC Genomics. 2016 Aug 11;17(1):619
pubmed: 27515280
PPAR Res. 2008;2008:172676
pubmed: 18815630
Front Endocrinol (Lausanne). 2015 Jan 19;5:241
pubmed: 25646091
Int J Cardiol. 2004 Dec;97(3):485-93
pubmed: 15561337
Horm Behav. 1978 Dec;11(3):323-47
pubmed: 112023
Development. 1999 Aug;126(16):3509-21
pubmed: 10409498
Sci Immunol. 2016 Aug;1(2):
pubmed: 28331908
Endocrinology. 1973 Mar;92(3):762-8
pubmed: 4701480
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
J Neuroimmunol. 2006 May;174(1-2):82-91
pubmed: 16530273
PLoS Genet. 2011 Jul;7(7):e1002201
pubmed: 21829381
Biol Pharm Bull. 2016;39(4):587-92
pubmed: 27040631
PLoS One. 2010 Jun 24;5(6):e11302
pubmed: 20585581
J Exerc Nutrition Biochem. 2016 Jun;20(2):51-7
pubmed: 27508154
Curr Opin Lipidol. 2004 Aug;15(4):393-8
pubmed: 15243211