Multiple recombination events between field and vaccine strains resulted in the emergence of a novel infectious bronchitis virus with decreased pathogenicity and altered replication capacity.
Animals
Antigens, Viral
/ analysis
Chickens
China
Coronavirus Infections
/ veterinary
Infectious bronchitis virus
/ genetics
Poultry Diseases
/ virology
Recombination, Genetic
Retrospective Studies
Serogroup
Specific Pathogen-Free Organisms
Vaccines, Attenuated
/ analysis
Viral Vaccines
/ analysis
Virulence
Virus Replication
antigenicity
genotype and lineage
infectious bronchitis virus
pathogenicity
recombination
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
22
09
2019
revised:
22
11
2019
accepted:
22
11
2019
entrez:
4
4
2020
pubmed:
4
4
2020
medline:
21
10
2020
Statut:
ppublish
Résumé
In this study, we isolated and identified 2 infectious bronchitis virus (IBV) strains from layer chickens soon after vaccination with the Massachusetts-Connecticut bivalent vaccine (Conn) and H120 and 4/91 booster vaccines in China in 2011. The results of cross-virus-neutralization tests and phylogenetic analysis of the S1 subunit of spike gene of these vaccine strains and other reference strains showed that strain LJL/110302 was of GI-19 lineage, whereas LLN/111169 was of the GI-1 lineage of the Conn serotype. Further comparative genomic analysis revealed that LLN/111169, an IBV strain with novel traits, originated from multiple recombination events (at least 3 recombination sites) between GI-19 and the Conn and 4/91 vaccine strains. LLN/111169 was pathogenic to specific pathogen-free (SPF) chickens. This is of prime importance because while IBV prevention measures worldwide are mainly dependent on modified live vaccine strains, our results showed that recombination between field and vaccine strains has produced a novel pathogenic IBV strain. In addition, LLN/111169 showed relatively broad tissue tropism (trachea, lungs, kidneys, and cecal tonsils) in infected SPF chickens. These results emphasize the importance of IBV surveillance in chicken flocks.
Identifiants
pubmed: 32241473
pii: S0032-5791(19)58057-9
doi: 10.1016/j.psj.2019.11.056
pmc: PMC7102566
pii:
doi:
Substances chimiques
Antigens, Viral
0
Vaccines, Attenuated
0
Viral Vaccines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1928-1938Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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