Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling.
Aquaporin 1
/ metabolism
Cells, Cultured
Extracellular Signal-Regulated MAP Kinases
/ metabolism
Human Umbilical Vein Endothelial Cells
/ enzymology
Humans
Hydrostatic Pressure
Mechanotransduction, Cellular
Neovascularization, Physiologic
Phosphorylation
Protein Kinase C-alpha
/ metabolism
Receptors, G-Protein-Coupled
/ metabolism
Water
/ metabolism
ras Proteins
/ metabolism
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
02 04 2020
02 04 2020
Historique:
received:
09
12
2018
accepted:
11
03
2020
entrez:
4
4
2020
pubmed:
4
4
2020
medline:
16
6
2021
Statut:
epublish
Résumé
Vascular tubulogenesis is tightly linked with physiological and pathological events in the living body. Endothelial cells (ECs), which are constantly exposed to hemodynamic forces, play a key role in tubulogenesis. Hydrostatic pressure in particular has been shown to elicit biophysical and biochemical responses leading to EC-mediated tubulogenesis. However, the relationship between tubulogenesis and hydrostatic pressure remains to be elucidated. Here, we propose a specific mechanism through which hydrostatic pressure promotes tubulogenesis. We show that pressure exposure transiently activates the Ras/extracellular signal-regulated kinase (ERK) pathway in ECs, inducing endothelial tubulogenic responses. Water efflux through aquaporin 1 and activation of protein kinase C via specific G protein-coupled receptors are essential to the pressure-induced transient activation of the Ras/ERK pathway. Our approach could provide a basis for elucidating the mechanopathology of tubulogenesis-related diseases and the development of mechanotherapies for improving human health.
Identifiants
pubmed: 32242084
doi: 10.1038/s42003-020-0881-9
pii: 10.1038/s42003-020-0881-9
pmc: PMC7118103
doi:
Substances chimiques
AQP1 protein, human
0
Receptors, G-Protein-Coupled
0
Water
059QF0KO0R
Aquaporin 1
146410-94-8
PRKCA protein, human
EC 2.7.11.13
Protein Kinase C-alpha
EC 2.7.11.13
Extracellular Signal-Regulated MAP Kinases
EC 2.7.11.24
ras Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
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