Serial block-face scanning electron microscopy reveals novel intercellular connections in human term placental microvasculature.
endothelial junction
filopodia
microvasculature
placenta
villi
Journal
Journal of anatomy
ISSN: 1469-7580
Titre abrégé: J Anat
Pays: England
ID NLM: 0137162
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
18
10
2019
revised:
03
03
2020
accepted:
05
03
2020
pubmed:
4
4
2020
medline:
2
4
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
The placental microvasculature is a conduit for fetal blood allowing solute exchange between the mother and the fetus. Serial block-face scanning electron microscopy (SBF SEM) allows ultrastructure to be viewed in three dimensions and provides a new perspective on placental anatomy. This study used SBF SEM to study endothelial cells within the human placental microvasculature from uncomplicated pregnancies. Term human placental villi were aldehyde-fixed and processed for imaging by SBF SEM. Manual segmentation was carried out on a terminal villous capillary and an intermediate villous arteriole and venule. Twenty-seven SBF SEM stacks from terminal villi were analysed using stereological approaches to determine the volumes of microvascular components and the proportions of pericyte coverage. SBF SEM analysis of capillary endothelial cells revealed the presence of interendothelial protrusions (IEPs) originating from the donor cell at the endothelial junction and forming deep thin projections up to 7 μm into the adjacent endothelial cells. IEP density was estimated to be in the order of 35 million cm
Identifiants
pubmed: 32242928
doi: 10.1111/joa.13191
pmc: PMC7369196
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
241-249Subventions
Organisme : Medical Research Council
ID : MR/L012626/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.
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