From Cord to Eye: Wharton Jelly-Derived Stem Cells Differentiate Into Corneal Endothelial-Like Cells.
Journal
Cornea
ISSN: 1536-4798
Titre abrégé: Cornea
Pays: United States
ID NLM: 8216186
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
pubmed:
4
4
2020
medline:
27
4
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
A malfunction of the corneal endothelium leading to corneal opacity is one of the main causes of impaired vision. Currently, keratoplasty is the one and only donor cornea-dependent treatment, and this calls for alternatives because of the worldwide lack of donor corneas. Recently, the topography of Descemet membrane (DM) has been discovered as a feasible stem cell differentiation tool. With this study, we further confirm this mechanotransductive system by using preinduced Wharton jelly-derived mesenchymal stem cells (WJ-EPCs). To measure the mechanotransductive potential of Descemet-like topography (DLT), WJ-EPCs were cultivated on collagen imprints with DLT. Changes in the gene and protein expressions of corneal endothelial cells (CECs), typical markers such as zonula occludens (ZO-1), sodium/potassium (Na/K)-ATPase, paired-like homeodomain 2 (PITX2), and collagen 8 (COL-8) were measured. In addition, CEC functionality has been evaluated by measuring the relative potential differences in a 2-compartment system and by measuring corneal transparency in an ex vivo rabbit cornea model. To confirm the activity of WJ-EPCs, rabbit CECs were restless deleted by collagen digestion of a thin layer of rabbit Descemet membrane. The proper CEC-typical hexagonal morphology of WJ-EPCs in combination with a significant expression of ZO-1, Na/K-ATPase, PITX2, and COL-8 could be demonstrated. In addition, the WJ-EPCs were able to build up a relative potential difference of 40 mV and to keep corneas clear and transparent. These data indicate that a well-characterized, functional CEC monolayer was developed by using a DLT-mediated mechanotransductive differentiation of WJ-EPCs.
Identifiants
pubmed: 32243419
doi: 10.1097/ICO.0000000000002319
pii: 00003226-202007000-00014
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
877-885Références
Ehlers N. Cornea in organ culture. Curr Opin Ophthalmol. 1990;1:354–359.
Bourne WM. Biology of the corneal endothelium in health and disease. Eye. 2003;17:912–918.
Dapena I, Ham L, Melles GRJ. Endothelial keratoplasty: DSEK/DSAEK or DMEK—the thinner the better? Curr Opin Ophthalmol. 2009;20:299–307.
Koo EH. A modified surgical technique for Descemet's stripping automated endothelial keratoplasty (DSAEK) in altered or abnormal anatomy. Am J Ophthalmol Case Rep. 2019;15:100497.
Chen X, Wu L, Li Z, et al. Directed differentiation of human corneal endothelial cells from human embryonic stem cells by using cell-conditioned culture media. IOVS. 2018;59:3028–3036.
Ali M, Khan SY, Vasanth S, et al. Generation and proteome profiling of PBMC-originated, iPSC-derived corneal endothelial cells. IOVS. 2018;59:2437–2444.
Shen L, Sun P, Zhang C, et al. Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors. Sci Rep. 2017;7:13400.
Inagaki E, Hatou S, Higa K, et al. Skin-derived precursors as a source of progenitors for corneal endothelial regeneration. Stem Cell Transl Med. 2017;6:788–798.
Yamashita K, Inagaki E, Hatou S, et al. Corneal endothelial regeneration using mesenchymal stem cells derived from human umbilical cord. Stem Cells Dev. 2018;27:1097–1108.
Le HQ, Ghatak S, Yeung CYC, et al. Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment. Nat Cell Biol. 2016;18:864–875.
Maurer M, Lammerding J. The driving force: nuclear mechanotransduction in cellular function, fate, and disease. Annu Rev Biomed Eng. 2019;21:443–468.
McMurray RJ, Dalby MJ, Tsimbouri PM. Using biomaterials to study stem cell mechanotransduction, growth and differentiation. J Tissue Eng Regen. 2015;9:528–539.
Chen S, Liu X, Wang N, et al. Visualizing micro-anatomical structures of the posterior cornea with micro-optical coherence tomography. Sci Rep. 2017;7:10752.
Gutermuth A, Maassen J, Harnisch E, et al. Descemet's membrane biomimetic microtopography differentiates human mesenchymal stem cells into corneal endothelial-like cells. Cornea. 2019;38:110–119.
Goebel M. Management of coronal tooth fractures. Northwest Dent. 1991;70:57–58.
Klimczak A, Kozlowska U. Mesenchymal stromal cells and tissue-specific progenitor cells: their role in tissue homeostasis. Stem Cell Int. 2016;2016:4285215.
Chandramohan A, Stinnett SS, Petrowski JT, et al. Visual function measures IN early and intermediate age-related macular degeneration. Retina. 2016;36:1021–1031.
Nanaev AK, Kohnen G, Milovanov AP, et al. Stromal differentiation and architecture of the human umbilical cord. Placenta. 1997;18:53–64.
Phipps RP, Penney DP, Keng P, et al. Characterization of two major populations of lung fibroblasts: distinguishing morphology and discordant display of Thy 1 and class II MHC. Am J Resp Cell Mol. 1989;1:65–74.
Hundt HK, Steyn JM, Wagner L. Post-mortem serum concentration of cantharidin in a fatal case of cantharides poisoning. Hum Exp Toxicol. 1990;9:35–40.
Chen L, Martino V, Dombkowski A, et al. AP-2β is a downstream effector of PITX2 required to specify endothelium and establish angiogenic privilege during corneal development. IOVS. 2016;57:1072–1081.