Cellular Stress Upregulates Indole Signaling Metabolites in Escherichia coli.
E. coli
IL-6
antibiotic tolerance
host-bacteria interactions
indole
innate immunity
persister cell
signaling
stress response
Journal
Cell chemical biology
ISSN: 2451-9448
Titre abrégé: Cell Chem Biol
Pays: United States
ID NLM: 101676030
Informations de publication
Date de publication:
18 06 2020
18 06 2020
Historique:
received:
18
12
2019
revised:
04
02
2020
accepted:
02
03
2020
pubmed:
4
4
2020
medline:
7
7
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
Escherichia coli broadly colonize the intestinal tract of humans and produce a variety of small molecule signals. However, many of these small molecules remain unknown. Here, we describe a family of widely distributed bacterial metabolites termed the "indolokines." In E. coli, the indolokines are upregulated in response to a redox stressor via aspC and tyrB transaminases. Although indolokine 1 represents a previously unreported metabolite, four of the indolokines (2-5) were previously shown to be derived from indole-3-carbonyl nitrile (ICN) in the plant pathogen defense response. We show that the indolokines are produced in a convergent evolutionary manner relative to plants, enhance E. coli persister cell formation, outperform ICN protection in an Arabidopsis thaliana-Pseudomonas syringae infection model, trigger a hallmark plant innate immune response, and activate distinct immunological responses in primary human tissues. Our molecular studies link a family of cellular stress-induced metabolites to defensive responses across bacteria, plants, and humans.
Identifiants
pubmed: 32243812
pii: S2451-9456(20)30077-5
doi: 10.1016/j.chembiol.2020.03.003
pmc: PMC7306003
mid: NIHMS1580180
pii:
doi:
Substances chimiques
Indoles
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
698-707.e7Subventions
Organisme : NCI NIH HHS
ID : DP2 CA186575
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007499
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA215553
Pays : United States
Organisme : EPA
ID : EP-W-17-011
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests R.W., T.P.W., and G.P. are employees of Merck Exploratory Science Center, Merck & Co., Inc., Kenilworth, NJ, USA. Employees may hold stocks and/or stock options in Merck & Co., Inc., Kenilworth, NJ, USA.
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