Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot.
autophagy
biomarkers
disease
pathology
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
21 Mar 2020
21 Mar 2020
Historique:
received:
02
03
2020
revised:
18
03
2020
accepted:
19
03
2020
entrez:
5
4
2020
pubmed:
5
4
2020
medline:
5
4
2020
Statut:
epublish
Résumé
Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.
Identifiants
pubmed: 32245178
pii: biology9030059
doi: 10.3390/biology9030059
pmc: PMC7150830
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Bernese Cancer League
ID : none
Organisme : Stiftung für klinisch-experimentelle Tumorforschung
ID : none
Organisme : Werner and Hedy Berger-Janser Foundation for Cancer Research
ID : none
Organisme : FIS and FEDER funds from the EU
ID : PI14/01085 and PI17/00093
Organisme : ISCIII and FSE funds
ID : CPII16/00023
Organisme : the Spanish Minsitry of Science, Innovation and Universities
ID : RTI2018-096748-B-100
Organisme : University Professor Training Fellowship, Ministry of Science, Innovation and University, Government of Spain
ID : none
Organisme : the State Plan for R & D + I2013-2016 and funded by the Instituto de Salud Carlos III
ID : PI18/00442
Organisme : European Regional Development Fund
ID : none
Organisme : Luxembourg National Research Fund
ID : C18/BM/12670304/COMBATIC
Organisme : Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, by the European Regional Development Fund (FEDER), through the Competitiveness Factors Operational Programme (COMPETE)
ID : NORTE-01-0145-FEDER-000013
Organisme : FEDER, through the COMPETE
ID : POCI-01-0145-FEDER-028159 and POCI-01-0145-FEDER-030782
Organisme : National funds, through the Foundation for Science and Technology (FCT
ID : none
Organisme : ARRS - the Slovenian research agency, programme P1-0140: Proteolysis and its regulation
ID : none
Organisme : the Swiss Cancer Research
ID : KFS-3360-02-2014
Organisme : the Swiss Cancer Research
ID : KFS-3409-02-2014
Organisme : Swiss National Science Foundation
ID : 31003A_173219
Pays : Switzerland
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