Metagenome sequencing to unveil the resistome in a deep subtropical lake on the Yunnan-Guizhou Plateau, China.

Anti-Bacterial Agents / pharmacology China Drug Resistance, Microbial / drug effects Genes, Bacterial / drug effects Lakes Metagenome / drug effects RNA, Ribosomal, 16S
Bacterial community Erhai lake Metagenomic analysis Resistance genes Spatial analysis

Journal

Environmental pollution (Barking, Essex : 1987)
ISSN: 1873-6424
Titre abrégé: Environ Pollut
Pays: England
ID NLM: 8804476

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 24 11 2019
revised: 23 03 2020
accepted: 25 03 2020
pubmed: 5 4 2020
medline: 11 7 2020
entrez: 5 4 2020
Statut: ppublish

Résumé

Erhai Lake in the Yunnan-Guizhou Plateau, a typical subtropical deep lake, experienced long-time input of nutrients and pollutants, which provides beneficial conditions for evolution and spread of resistance genes. In this study, the profile of bacterial community, antibiotic and metal resistance genes in sediments of Erhai Lake were analyzed via high-thought sequencing of 16S rRNA and metagenomic DNA. Proteobacteria, Firmicutes, Nitrospirae, and Bacteroidetes were found to be the main composition of the bacterial community. Multidrug-, bacitracin-, macrolide-lincosamide-streptogramin (MLS)- and tetracycline resistance genes were the primary antibiotic resistance gene (ARG) types with high relative abundance, whereas Multi-metal-, and arsenic- resistance genes were the primary metal resistance gene (MRG) types. The effects of nitrogen and phosphorus on the abundance of ARGs and MRGs depended on the different types. Some specific ARG (fosmidomycin resistance genes) and MRG types (multi-metal resistance genes) also showed significant geographic distribution. Bacterial community was the main driver shaping the resistome. Nutrients also played an essential role in structuring the bacterial community and resistome in the sediments of Erhai Lake. This study sheds light on the distribution and fate of resistome under a high load of nitrogen and phosphorus in a deep lake.

Identifiants

DOI: 10.1016/j.envpol.2020.114470 PMID: 32247114
pubmed: 32247114
pii: S0269-7491(19)36995-7
doi: 10.1016/j.envpol.2020.114470
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

114470

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Xiong Pan (X)

Basin Water Environmental Research Department, Changjiang River Scientific Research Institute, Wuhan, 430014, China.

Li Lin (L)

Basin Water Environmental Research Department, Changjiang River Scientific Research Institute, Wuhan, 430014, China.

Weihong Zhang (W)

Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Center of the Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, 430074, China.

Lei Dong (L)

Basin Water Environmental Research Department, Changjiang River Scientific Research Institute, Wuhan, 430014, China.

Yuyi Yang (Y)

Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Center of the Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, 430074, China. Electronic address: yangyy@wbgcas.cn.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Régions géographiques › Asie › Extrême-Orient › Chine › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Gènes bactériens › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Environnement et santé publique › Environnement › Eau douce › Lacs › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Métagénome › Metagenome sequencing to unveil the resistome...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Metagenome sequencing to unveil the resistome...