Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept.
Fast field-cycling magnetic resonance
Inflammation
Ultrasmall superparamagnetic iron oxide particles (USPIO)
Journal
Journal of magnetic resonance (San Diego, Calif. : 1997)
ISSN: 1096-0856
Titre abrégé: J Magn Reson
Pays: United States
ID NLM: 9707935
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
22
11
2019
revised:
19
03
2020
accepted:
20
03
2020
pubmed:
6
4
2020
medline:
13
10
2021
entrez:
6
4
2020
Statut:
ppublish
Résumé
Inflammation is central in disease pathophysiology and accurate methods for its detection and quantification are increasingly required to guide diagnosis and therapy. Here we explored the ability of Fast Field-Cycling Magnetic Resonance (FFC-MR) in quantifying the signal of ultra-small superparamagnetic iron oxide particles (USPIO) phagocytosed by J774 macrophage-like cells as a proof-of-principle. Relaxation rates were measured in suspensions of J774 macrophage-like cells loaded with USPIO (0-200 μg/ml Fe as ferumoxytol), using a 0.25 T FFC benchtop relaxometer and a human whole-body, in-house built 0.2 T FFC-MR prototype system with a custom test tube coil. Identical non-imaging, saturation recovery pulse sequence with 90° flip angle and 20 different evolution fields selected logarithmically between 80 μT and 0.2 T (3.4 kHz and 8.51 MHz proton Larmor frequency [PLF] respectively). Results were compared with imaging flow cytometry quantification of side scatter intensity and USPIO-occupied cell area. A reference colorimetric iron assay was used. The T We demonstrated for the first time that FFC-MR is capable of quantitative assessment of intra-cellular iron which will have important implications for the use of USPIO in a variety of biological applications, including the study of inflammation.
Identifiants
pubmed: 32248086
pii: S1090-7807(20)30040-9
doi: 10.1016/j.jmr.2020.106722
pmc: PMC7167511
pii:
doi:
Substances chimiques
Suspensions
0
Ferrosoferric Oxide
XM0M87F357
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106722Subventions
Organisme : British Heart Foundation
ID : PG/15/108/31928
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest DJL, DKD, HMW, RY, LC, PJR, LB, AE, DL and HA have no conflicts of interest to declare.
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