Overexpression of miR-199/214 is a distinctive feature of iron-induced and asbestos-induced sarcomatoid mesothelioma in rats.
Twist1
animal model
asbestos
iron-induced mesothelioma
miR-199/214
Journal
Cancer science
ISSN: 1349-7006
Titre abrégé: Cancer Sci
Pays: England
ID NLM: 101168776
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
03
12
2019
revised:
17
03
2020
accepted:
24
03
2020
pubmed:
6
4
2020
medline:
20
6
2020
entrez:
6
4
2020
Statut:
ppublish
Résumé
Malignant mesothelioma (MM) is one of the most lethal tumors in humans. The onset of MM is linked to exposure to asbestos, which generates reactive oxygen species (ROS). ROS are believed to be derived from the frustrated phagocytosis and the iron in asbestos. To explore the pathogenesis of MM, peritoneal MM was induced in rats by the repeated intraperitoneal injection of iron saccharate and nitrilotriacetate. In the present study, we used microarray techniques to screen the microRNA (miR) expression profiles of these MM. We observed that the histological subtype impacted the hierarchical clustering of miR expression profiles and determined that miR-199/214 is a distinctive feature of iron saccharate-induced sarcomatoid mesothelioma (SM). Twist1, a transcriptional regulator of the epithelial-mesenchymal transition, has been shown to activate miR-199/214 transcription; thus, the expression level of Twist1 was examined in iron-induced and asbestos-induced mesotheliomas in rats. Twist1 was exclusively expressed in iron saccharate-induced SM but not in the epithelioid subtype. The Twist1-miR-199/214 axis is activated in iron saccharate-induced and asbestos-induced SM. The expression levels of miR-214 and Twist1 were correlated in an asbestos-induced MM cell line, suggesting that the Twist1-miR-199/214 axis is preserved. MeT5A, an immortalized human mesothelial cell line, was used for the functional analysis of miR. The overexpression of miR-199/214 promoted cellular proliferation, mobility and phosphorylation of Akt and ERK in MeT5A cells. These results indicate that miR-199/214 may affect the aggressive biological behavior of SM.
Identifiants
pubmed: 32248600
doi: 10.1111/cas.14405
pmc: PMC7293088
doi:
Substances chimiques
MIRN199 microRNA, rat
0
MIRN214 microRNA, human
0
MicroRNAs
0
Mirn214 microRNA, rat
0
Twist-Related Protein 1
0
mirn199 microRNA, human
0
Asbestos
1332-21-4
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2016-2027Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology
Organisme : Japan Society for the Promotion of Science
ID : Young Scientist (B)(23790440)
Organisme : Japan Society for the Promotion of Science
ID : Young Scientist (B)(25860292)
Organisme : JSPS Kakenhi
ID : JP17H04064
Organisme : JSPS Kakenhi
ID : JP19H05462
Organisme : JST CREST
ID : JPMJCR19H4
Informations de copyright
© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
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