Unique hexameric structure of copper-containing nitrite reductase of an anammox bacterium KSU-1.
Anammox
Copper-containing nitrite reductase
Electron transfer
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
04 06 2020
04 06 2020
Historique:
received:
22
03
2020
accepted:
25
03
2020
pubmed:
7
4
2020
medline:
15
12
2020
entrez:
7
4
2020
Statut:
ppublish
Résumé
Anaerobic ammonium oxidation (anammox) and denitrification are two different microbial reactions that form nitrogen gas. The initial step in the anammox reaction-reduction of nitrite to nitric oxide-is thought to be catalyzed by homologs of dissimilatory nitrite reductase, which is known to be involved in denitrification. Here, we reveal the crystal structure of the copper-containing nitrite reductase (CuNIR) of strain KSU-1, an anammox bacterium. CuNIR had a unique homohexameric structure with three disulfide bridges between homotrimers, although the trimer was similar to that of known CuNIRs. Kinetic and mutagenesis analyses suggested that the hexameric structure is important for the electron transfer reaction.
Identifiants
pubmed: 32248970
pii: S0006-291X(20)30641-0
doi: 10.1016/j.bbrc.2020.03.144
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Nitrite Reductases
EC 1.7.-
nitrite reductase, copper-containing
EC 1.7.2.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
654-660Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.