Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells.
conditioned medium
dog
mesenchymal stromal cell
neural differentiation
Journal
The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360
Informations de publication
Date de publication:
20 May 2020
20 May 2020
Historique:
pubmed:
7
4
2020
medline:
7
1
2021
entrez:
7
4
2020
Statut:
ppublish
Résumé
We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70-80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.
Identifiants
pubmed: 32249241
doi: 10.1292/jvms.19-0141
pmc: PMC7273601
doi:
Substances chimiques
Culture Media, Conditioned
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
668-672Références
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